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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodopseudomonas palustris Biotin synthase (bioB) CSB-YP461302RLV
CSB-EP461302RLV
CSB-BP461302RLV
CSB-MP461302RLV
CSB-EP461302RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris PKHD-type hydroxylase Rpal_3968 (Rpal_3968) CSB-YP461303RLV
CSB-EP461303RLV
CSB-BP461303RLV
CSB-MP461303RLV
CSB-EP461303RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP461304RLV
CSB-EP461304RLV
CSB-BP461304RLV
CSB-MP461304RLV
CSB-EP461304RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Urease accessory protein UreE (ureE) CSB-YP461305RLV
CSB-EP461305RLV
CSB-BP461305RLV
CSB-MP461305RLV
CSB-EP461305RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP461306RLV
CSB-EP461306RLV
CSB-BP461306RLV
CSB-MP461306RLV
CSB-EP461306RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Alkanesulfonate monooxygenase (ssuD) CSB-YP461307RLV
CSB-EP461307RLV
CSB-BP461307RLV
CSB-MP461307RLV
CSB-EP461307RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Bifunctional protein HldE (hldE) CSB-YP461308RLV
CSB-EP461308RLV
CSB-BP461308RLV
CSB-MP461308RLV
CSB-EP461308RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP461309RLV
CSB-EP461309RLV
CSB-BP461309RLV
CSB-MP461309RLV
CSB-EP461309RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Probable malate:quinone oxidoreductase (mqo) CSB-YP461310RLV
CSB-EP461310RLV
CSB-BP461310RLV
CSB-MP461310RLV
CSB-EP461310RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Malate synthase G (glcB), partial CSB-YP461311RLV
CSB-EP461311RLV
CSB-BP461311RLV
CSB-MP461311RLV
CSB-EP461311RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Phosphoheptose isomerase (gmhA) CSB-YP461312DSQ
CSB-EP461312DSQ
CSB-BP461312DSQ
CSB-MP461312DSQ
CSB-EP461312DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 50S ribosomal protein L13 (rplM) CSB-YP461313DSQ
CSB-EP461313DSQ
CSB-BP461313DSQ
CSB-MP461313DSQ
CSB-EP461313DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP461314DSQ
CSB-EP461314DSQ
CSB-BP461314DSQ
CSB-MP461314DSQ
CSB-EP461314DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Deoxyhypusine synthase-like protein (Cpar_2043) CSB-YP461315DSQ
CSB-EP461315DSQ
CSB-BP461315DSQ
CSB-MP461315DSQ
CSB-EP461315DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Indole-3-glycerol phosphate synthase (trpC) CSB-YP461316DSQ
CSB-EP461316DSQ
CSB-BP461316DSQ
CSB-MP461316DSQ
CSB-EP461316DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Tyrosine recombinase XerC (xerC) CSB-YP461317DSQ
CSB-EP461317DSQ
CSB-BP461317DSQ
CSB-MP461317DSQ
CSB-EP461317DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Anthranilate phosphoribosyltransferase (trpD) CSB-YP461318DSQ
CSB-EP461318DSQ
CSB-BP461318DSQ
CSB-MP461318DSQ
CSB-EP461318DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum UPF0758 protein Cpar_0627 (Cpar_0627) CSB-YP461319DSQ
CSB-EP461319DSQ
CSB-BP461319DSQ
CSB-MP461319DSQ
CSB-EP461319DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum UPF0133 protein Cpar_0834 (Cpar_0834) CSB-YP461320DSQ
CSB-EP461320DSQ
CSB-BP461320DSQ
CSB-MP461320DSQ
CSB-EP461320DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Protein translocase subunit SecA (secA), partial CSB-YP461321DSQ
CSB-EP461321DSQ
CSB-BP461321DSQ
CSB-MP461321DSQ
CSB-EP461321DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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