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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chloroherpeton thalassium Lon protease (lon), partial CSB-YP461341DSY
CSB-EP461341DSY
CSB-BP461341DSY
CSB-MP461341DSY
CSB-EP461341DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP461342DSY
CSB-EP461342DSY
CSB-BP461342DSY
CSB-MP461342DSY
CSB-EP461342DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP461343DSY
CSB-EP461343DSY
CSB-BP461343DSY
CSB-MP461343DSY
CSB-EP461343DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP461344DSY
CSB-EP461344DSY
CSB-BP461344DSY
CSB-MP461344DSY
CSB-EP461344DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Dihydrodipicolinate synthase (dapA) CSB-YP461345DSY
CSB-EP461345DSY
CSB-BP461345DSY
CSB-MP461345DSY
CSB-EP461345DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Thiazole synthase (thiG) CSB-YP461346DSY
CSB-EP461346DSY
CSB-BP461346DSY
CSB-MP461346DSY
CSB-EP461346DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP461347DSY
CSB-EP461347DSY
CSB-BP461347DSY
CSB-MP461347DSY
CSB-EP461347DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium DNA mismatch repair protein mutL (mutL), partial CSB-YP461348DSY
CSB-EP461348DSY
CSB-BP461348DSY
CSB-MP461348DSY
CSB-EP461348DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP461349DSY
CSB-EP461349DSY
CSB-BP461349DSY
CSB-MP461349DSY
CSB-EP461349DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Glutamyl-tRNA reductase (hemA) CSB-YP461350DSY
CSB-EP461350DSY
CSB-BP461350DSY
CSB-MP461350DSY
CSB-EP461350DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP461351DSY
CSB-EP461351DSY
CSB-BP461351DSY
CSB-MP461351DSY
CSB-EP461351DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Ferredoxin--NADP reductase 1 (Ctha_0950) CSB-YP461352DSY
CSB-EP461352DSY
CSB-BP461352DSY
CSB-MP461352DSY
CSB-EP461352DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP461353DSY
CSB-EP461353DSY
CSB-BP461353DSY
CSB-MP461353DSY
CSB-EP461353DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Hydroxylamine reductase (hcp), partial CSB-YP461354DSY
CSB-EP461354DSY
CSB-BP461354DSY
CSB-MP461354DSY
CSB-EP461354DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium 50S ribosomal protein L22 (rplV) CSB-YP461355DSY
CSB-EP461355DSY
CSB-BP461355DSY
CSB-MP461355DSY
CSB-EP461355DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium 50S ribosomal protein L29 (rpmC) CSB-YP461356DSY
CSB-EP461356DSY
CSB-BP461356DSY
CSB-MP461356DSY
CSB-EP461356DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium 30S ribosomal protein S8 (rpsH) CSB-YP461357DSY
CSB-EP461357DSY
CSB-BP461357DSY
CSB-MP461357DSY
CSB-EP461357DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium UPF0133 protein Ctha_1157 (Ctha_1157) CSB-YP461358DSY
CSB-EP461358DSY
CSB-BP461358DSY
CSB-MP461358DSY
CSB-EP461358DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP461359DSY
CSB-EP461359DSY
CSB-BP461359DSY
CSB-MP461359DSY
CSB-EP461359DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP461360DSY
CSB-EP461360DSY
CSB-BP461360DSY
CSB-MP461360DSY
CSB-EP461360DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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