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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lottia gigantea Uncharacterized shell protein 26 CSB-YP462902LQP
CSB-EP462902LQP
CSB-BP462902LQP
CSB-MP462902LQP
CSB-EP462902LQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lottia gigantea Uncharacterized shell protein 8 CSB-YP462903LQP
CSB-EP462903LQP
CSB-BP462903LQP
CSB-MP462903LQP
CSB-EP462903LQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lottia gigantea Threonine-rich protein CSB-YP462904LQP
CSB-EP462904LQP
CSB-BP462904LQP
CSB-MP462904LQP
CSB-EP462904LQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP462905WBO
CSB-EP462905WBO
CSB-BP462905WBO
CSB-MP462905WBO
CSB-EP462905WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Lon protease (lon), partial CSB-YP462906WBO
CSB-EP462906WBO
CSB-BP462906WBO
CSB-MP462906WBO
CSB-EP462906WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 50S ribosomal protein L13 (rplM) CSB-YP462907WBO
CSB-EP462907WBO
CSB-BP462907WBO
CSB-MP462907WBO
CSB-EP462907WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Heme A synthase (ctaA), partial CSB-YP462908WBO
CSB-EP462908WBO
CSB-BP462908WBO
CSB-MP462908WBO
CSB-EP462908WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens UPF0335 protein WP0746 (WP0746) CSB-YP462909WBO
CSB-EP462909WBO
CSB-BP462909WBO
CSB-MP462909WBO
CSB-EP462909WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens tRNA (Ile)-lysidine synthase CSB-YP462910WBO
CSB-EP462910WBO
CSB-BP462910WBO
CSB-MP462910WBO
CSB-EP462910WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Phosphoglycerate kinase (pgk) CSB-YP462911WBO
CSB-EP462911WBO
CSB-BP462911WBO
CSB-MP462911WBO
CSB-EP462911WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 50S ribosomal protein L19 (rplS) CSB-YP462912WBO
CSB-EP462912WBO
CSB-BP462912WBO
CSB-MP462912WBO
CSB-EP462912WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Phosphate acyltransferase (plsX) CSB-YP462913WBO
CSB-EP462913WBO
CSB-BP462913WBO
CSB-MP462913WBO
CSB-EP462913WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP462914WBO
CSB-EP462914WBO
CSB-BP462914WBO
CSB-MP462914WBO
CSB-EP462914WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 50S ribosomal protein L2 (rplB) CSB-YP462915WBO
CSB-EP462915WBO
CSB-BP462915WBO
CSB-MP462915WBO
CSB-EP462915WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 50S ribosomal protein L33 (rpmG) CSB-YP462916WBO
CSB-EP462916WBO
CSB-BP462916WBO
CSB-MP462916WBO
CSB-EP462916WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Chaperone protein DnaJ (dnaJ) CSB-YP462917WBO
CSB-EP462917WBO
CSB-BP462917WBO
CSB-MP462917WBO
CSB-EP462917WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens D-alanine--D-alanine ligase (ddl) CSB-YP462918WBO
CSB-EP462918WBO
CSB-BP462918WBO
CSB-MP462918WBO
CSB-EP462918WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens SsrA-binding protein (smpB) CSB-YP462919WBO
CSB-EP462919WBO
CSB-BP462919WBO
CSB-MP462919WBO
CSB-EP462919WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 60 kDa chaperonin (groL), partial CSB-YP462920OEV
CSB-EP462920OEV
CSB-BP462920OEV
CSB-MP462920OEV
CSB-EP462920OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L36 (rpmJ) CSB-YP462921OEV
CSB-EP462921OEV
CSB-BP462921OEV
CSB-MP462921OEV
CSB-EP462921OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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