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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Porphyromonas gingivalis Lys-gingipain (kgp), partial CSB-YP464342EXZ
CSB-BP464342EXZ
CSB-MP464342EXZ
CSB-EP464342EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 50S ribosomal protein L18 (rplR) CSB-YP464343EXZ
CSB-EP464343EXZ
CSB-BP464343EXZ
CSB-MP464343EXZ
CSB-EP464343EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 50S ribosomal protein L22 (rplV) CSB-YP464344EXZ
CSB-EP464344EXZ
CSB-BP464344EXZ
CSB-MP464344EXZ
CSB-EP464344EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 30S ribosomal protein S7 (rpsG) CSB-YP464345EXZ
CSB-EP464345EXZ
CSB-BP464345EXZ
CSB-MP464345EXZ
CSB-EP464345EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis UvrABC system protein C (uvrC), partial CSB-YP464346EXZ
CSB-EP464346EXZ
CSB-BP464346EXZ
CSB-MP464346EXZ
CSB-EP464346EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis tRNA (Ile)-lysidine synthase CSB-YP464347EXZ
CSB-EP464347EXZ
CSB-BP464347EXZ
CSB-MP464347EXZ
CSB-EP464347EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP464348EXZ
CSB-EP464348EXZ
CSB-BP464348EXZ
CSB-MP464348EXZ
CSB-EP464348EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C17orf105 (C17orf105) CSB-YP464349HU
CSB-EP464349HU
CSB-BP464349HU
CSB-MP464349HU
CSB-EP464349HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Heat shock factor-binding protein 1-like protein 1 (Hsbp1l1) CSB-YP464350MO
CSB-EP464350MO
CSB-BP464350MO
CSB-MP464350MO
CSB-EP464350MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human BTB/POZ domain-containing protein 18 (BTBD18) CSB-YP464351HU
CSB-EP464351HU
CSB-BP464351HU
CSB-MP464351HU
CSB-EP464351HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP464352BUJ
CSB-EP464352BUJ
CSB-BP464352BUJ
CSB-MP464352BUJ
CSB-EP464352BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii D-alanine--D-alanine ligase (ddl) CSB-YP464353BUJ
CSB-EP464353BUJ
CSB-BP464353BUJ
CSB-MP464353BUJ
CSB-EP464353BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP464354BUJ
CSB-EP464354BUJ
CSB-BP464354BUJ
CSB-MP464354BUJ
CSB-EP464354BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii 30S ribosomal protein S7 (rpsG) CSB-YP464355BUJ
CSB-EP464355BUJ
CSB-BP464355BUJ
CSB-MP464355BUJ
CSB-EP464355BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii 50S ribosomal protein L1 (rplA) CSB-YP464356BUJ
CSB-EP464356BUJ
CSB-BP464356BUJ
CSB-MP464356BUJ
CSB-EP464356BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii UPF0133 protein BH0462 (BH0462) CSB-YP464357BUJ
CSB-EP464357BUJ
CSB-BP464357BUJ
CSB-MP464357BUJ
CSB-EP464357BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii 30S ribosomal protein S19 (rpsS) CSB-YP464358BUJ
CSB-EP464358BUJ
CSB-BP464358BUJ
CSB-MP464358BUJ
CSB-EP464358BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii 30S ribosomal protein S17 (rpsQ) CSB-YP464359BUJ
CSB-EP464359BUJ
CSB-BP464359BUJ
CSB-MP464359BUJ
CSB-EP464359BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii 50S ribosomal protein L36 (rpmJ) CSB-YP464360BUJ
CSB-EP464360BUJ
CSB-BP464360BUJ
CSB-MP464360BUJ
CSB-EP464360BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Ribose-5-phosphate isomerase A (rpiA) CSB-YP464361BUJ
CSB-EP464361BUJ
CSB-BP464361BUJ
CSB-MP464361BUJ
CSB-EP464361BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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