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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Treponema pallidum subsp. pallidum ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP464382TPS
CSB-EP464382TPS
CSB-BP464382TPS
CSB-MP464382TPS
CSB-EP464382TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Elongation factor P (efp) CSB-YP464383TPS
CSB-EP464383TPS
CSB-BP464383TPS
CSB-MP464383TPS
CSB-EP464383TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Probable tRNA sulfurtransferase (thiI) CSB-YP464384TPS
CSB-EP464384TPS
CSB-BP464384TPS
CSB-MP464384TPS
CSB-EP464384TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Asparagine--tRNA ligase (asnS), partial CSB-YP464385TPS
CSB-EP464385TPS
CSB-BP464385TPS
CSB-MP464385TPS
CSB-EP464385TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Carbon storage regulator homolog (csrA) CSB-YP464386TPS
CSB-EP464386TPS
CSB-BP464386TPS
CSB-MP464386TPS
CSB-EP464386TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP464387TPS
CSB-EP464387TPS
CSB-BP464387TPS
CSB-MP464387TPS
CSB-EP464387TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum 50S ribosomal protein L32 (rpmF) CSB-YP464388TPS
CSB-EP464388TPS
CSB-BP464388TPS
CSB-MP464388TPS
CSB-EP464388TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP464389TPS
CSB-EP464389TPS
CSB-BP464389TPS
CSB-MP464389TPS
CSB-EP464389TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum tRNA pseudouridine synthase B (truB) CSB-YP464390TPS
CSB-EP464390TPS
CSB-BP464390TPS
CSB-MP464390TPS
CSB-EP464390TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP464391BWR
CSB-EP464391BWR
CSB-BP464391BWR
CSB-MP464391BWR
CSB-EP464391BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Integration host factor subunit alpha (ihfA) CSB-YP464392BWR
CSB-EP464392BWR
CSB-BP464392BWR
CSB-MP464392BWR
CSB-EP464392BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP464393BWR
CSB-EP464393BWR
CSB-BP464393BWR
CSB-MP464393BWR
CSB-EP464393BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP464394BWR
CSB-EP464394BWR
CSB-BP464394BWR
CSB-MP464394BWR
CSB-EP464394BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Glutamate racemase (murI) CSB-YP464395BWR
CSB-EP464395BWR
CSB-BP464395BWR
CSB-MP464395BWR
CSB-EP464395BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L24 (rplX) CSB-YP464396BWR
CSB-EP464396BWR
CSB-BP464396BWR
CSB-MP464396BWR
CSB-EP464396BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 30S ribosomal protein S3 (rpsC) CSB-YP464397BWR
CSB-EP464397BWR
CSB-BP464397BWR
CSB-MP464397BWR
CSB-EP464397BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 30S ribosomal protein S10 (rpsJ) CSB-YP464398BWR
CSB-EP464398BWR
CSB-BP464398BWR
CSB-MP464398BWR
CSB-EP464398BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L7/L12 (rplL) CSB-YP464399BWR
CSB-EP464399BWR
CSB-BP464399BWR
CSB-MP464399BWR
CSB-EP464399BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus DNA ligase (ligA), partial CSB-YP464400BWR
CSB-EP464400BWR
CSB-BP464400BWR
CSB-MP464400BWR
CSB-EP464400BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP464401BWR1
CSB-EP464401BWR1
CSB-BP464401BWR1
CSB-MP464401BWR1
CSB-EP464401BWR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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