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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum Glutamate--tRNA ligase (gltX) CSB-YP464542DUG
CSB-EP464542DUG
CSB-BP464542DUG
CSB-MP464542DUG
CSB-EP464542DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum V-type ATP synthase subunit D (atpD) CSB-YP464543DUG
CSB-EP464543DUG
CSB-BP464543DUG
CSB-MP464543DUG
CSB-EP464543DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Trigger factor (tig) CSB-YP464544DUG
CSB-EP464544DUG
CSB-BP464544DUG
CSB-MP464544DUG
CSB-EP464544DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP464545DUG
CSB-EP464545DUG
CSB-BP464545DUG
CSB-MP464545DUG
CSB-EP464545DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Shikimate kinase (aroK) CSB-YP464546DUG
CSB-EP464546DUG
CSB-BP464546DUG
CSB-MP464546DUG
CSB-EP464546DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0042 nucleotide-binding protein CLL_A3342 (CLL_A3342) CSB-YP464547DUG
CSB-EP464547DUG
CSB-BP464547DUG
CSB-MP464547DUG
CSB-EP464547DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0133 protein CLL_A3446 (CLL_A3446) CSB-YP464548DUG
CSB-EP464548DUG
CSB-BP464548DUG
CSB-MP464548DUG
CSB-EP464548DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum N utilization substance protein B homolog (nusB) CSB-YP464549DUG
CSB-EP464549DUG
CSB-BP464549DUG
CSB-MP464549DUG
CSB-EP464549DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L35 (rpmI) CSB-YP464550DUG
CSB-EP464550DUG
CSB-BP464550DUG
CSB-MP464550DUG
CSB-EP464550DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Enterobactin exporter EntS (entS), partial CSB-YP464551SYZ
CSB-EP464551SYZ
CSB-BP464551SYZ
CSB-MP464551SYZ
CSB-EP464551SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0502 protein YceH (yceH) CSB-YP464552SYZ
CSB-EP464552SYZ
CSB-BP464552SYZ
CSB-MP464552SYZ
CSB-EP464552SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Curved DNA-binding protein (cbpA) CSB-YP464553SYZ
CSB-EP464553SYZ
CSB-BP464553SYZ
CSB-MP464553SYZ
CSB-EP464553SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Nucleoid occlusion factor SlmA (slmA) CSB-YP464554SYZ
CSB-EP464554SYZ
CSB-BP464554SYZ
CSB-MP464554SYZ
CSB-EP464554SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) CSB-YP464555SYZ
CSB-EP464555SYZ
CSB-BP464555SYZ
CSB-MP464555SYZ
CSB-EP464555SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 LPS-assembly lipoprotein lptE (lptE) CSB-YP464556SYZ
CSB-EP464556SYZ
CSB-BP464556SYZ
CSB-MP464556SYZ
CSB-EP464556SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Octanoyltransferase (lipB) CSB-YP464557SYZ
CSB-EP464557SYZ
CSB-BP464557SYZ
CSB-MP464557SYZ
CSB-EP464557SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Chromosome partition protein mukF (mukF) CSB-YP464558SYZ
CSB-EP464558SYZ
CSB-BP464558SYZ
CSB-MP464558SYZ
CSB-EP464558SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP464559SYZ
CSB-EP464559SYZ
CSB-BP464559SYZ
CSB-MP464559SYZ
CSB-EP464559SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Ribonuclease P protein component (rnpA) CSB-YP464560SYZ
CSB-EP464560SYZ
CSB-BP464560SYZ
CSB-MP464560SYZ
CSB-EP464560SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Tyrosine recombinase XerC (xerC) CSB-YP464561SYZ
CSB-EP464561SYZ
CSB-BP464561SYZ
CSB-MP464561SYZ
CSB-EP464561SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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