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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xanthomonas oryzae pv. oryzae Acireductone dioxygenase (mtnD1) CSB-YP464462XBC
CSB-EP464462XBC
CSB-BP464462XBC
CSB-MP464462XBC
CSB-EP464462XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP464463XBC
CSB-EP464463XBC
CSB-BP464463XBC
CSB-MP464463XBC
CSB-EP464463XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP464464XBC
CSB-EP464464XBC
CSB-BP464464XBC
CSB-MP464464XBC
CSB-EP464464XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP464465XBC
CSB-EP464465XBC
CSB-BP464465XBC
CSB-MP464465XBC
CSB-EP464465XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Protein slyX homolog (slyX) CSB-YP464466XBC
CSB-EP464466XBC
CSB-BP464466XBC
CSB-MP464466XBC
CSB-EP464466XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Outer-membrane lipoprotein lolB (lolB) CSB-YP464467XBC
CSB-EP464467XBC
CSB-BP464467XBC
CSB-MP464467XBC
CSB-EP464467XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP464468XBC
CSB-EP464468XBC
CSB-BP464468XBC
CSB-MP464468XBC
CSB-EP464468XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 50S ribosomal protein L5 (rplE) CSB-YP464469XBC
CSB-EP464469XBC
CSB-BP464469XBC
CSB-MP464469XBC
CSB-EP464469XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 30S ribosomal protein S5 (rpsE) CSB-YP464470XBC
CSB-EP464470XBC
CSB-BP464470XBC
CSB-MP464470XBC
CSB-EP464470XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP464471XBC
CSB-EP464471XBC
CSB-BP464471XBC
CSB-MP464471XBC
CSB-EP464471XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Coenzyme PQQ synthesis protein D (pqqD) CSB-YP464472XBC
CSB-EP464472XBC
CSB-BP464472XBC
CSB-MP464472XBC
CSB-EP464472XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Protein TolB (tolB) CSB-YP464473XBC
CSB-EP464473XBC
CSB-BP464473XBC
CSB-MP464473XBC
CSB-EP464473XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Putative pterin-4-alpha-carbinolamine dehydratase (PXO_01523) CSB-YP464474XBC
CSB-EP464474XBC
CSB-BP464474XBC
CSB-MP464474XBC
CSB-EP464474XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP464475XBC
CSB-EP464475XBC
CSB-BP464475XBC
CSB-MP464475XBC
CSB-EP464475XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Protein RecA (recA) CSB-YP464476XBC
CSB-EP464476XBC
CSB-BP464476XBC
CSB-MP464476XBC
CSB-EP464476XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 50S ribosomal protein L35 (rpmI) CSB-YP464477XBC
CSB-EP464477XBC
CSB-BP464477XBC
CSB-MP464477XBC
CSB-EP464477XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Elongation factor P-like protein (PXO_00104) CSB-YP464478XBC
CSB-EP464478XBC
CSB-BP464478XBC
CSB-MP464478XBC
CSB-EP464478XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Leucine--tRNA ligase (leuS), partial CSB-YP464479XBC
CSB-EP464479XBC
CSB-BP464479XBC
CSB-MP464479XBC
CSB-EP464479XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Thiazole synthase (thiG) CSB-YP464480XBC
CSB-EP464480XBC
CSB-BP464480XBC
CSB-MP464480XBC
CSB-EP464480XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP464481BXU
CSB-EP464481BXU
CSB-BP464481BXU
CSB-MP464481BXU
CSB-EP464481BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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