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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella dublin 50S ribosomal protein L31 (rpmE) CSB-YP466343SWN
CSB-EP466343SWN
CSB-BP466343SWN
CSB-MP466343SWN
CSB-EP466343SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0145 protein ybjQ (ybjQ) CSB-YP466344SWN
CSB-EP466344SWN
CSB-BP466344SWN
CSB-MP466344SWN
CSB-EP466344SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Putative ion-transport protein yfeO (yfeO), partial CSB-YP466345SWN
CSB-EP466345SWN
CSB-BP466345SWN
CSB-MP466345SWN
CSB-EP466345SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Uracil phosphoribosyltransferase (upp) CSB-YP466346SWN
CSB-EP466346SWN
CSB-BP466346SWN
CSB-MP466346SWN
CSB-EP466346SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 50S ribosomal protein L7/L12 (rplL) CSB-YP466347SWN
CSB-EP466347SWN
CSB-BP466347SWN
CSB-MP466347SWN
CSB-EP466347SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin NADH pyrophosphatase (nudC) CSB-YP466348SWN
CSB-EP466348SWN
CSB-BP466348SWN
CSB-MP466348SWN
CSB-EP466348SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Chorismate--pyruvate lyase (ubiC) CSB-YP466349SWN
CSB-EP466349SWN
CSB-BP466349SWN
CSB-MP466349SWN
CSB-EP466349SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0319 protein YccT (yccT) CSB-YP466350SWN
CSB-EP466350SWN
CSB-BP466350SWN
CSB-MP466350SWN
CSB-EP466350SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Flavoprotein wrbA (wrbA) CSB-YP466351SWN
CSB-EP466351SWN
CSB-BP466351SWN
CSB-MP466351SWN
CSB-EP466351SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Chaperone protein hscA (hscA), partial CSB-YP466352SWN
CSB-EP466352SWN
CSB-BP466352SWN
CSB-MP466352SWN
CSB-EP466352SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Elongation factor 4 (lepA), partial CSB-YP466353SWN
CSB-EP466353SWN
CSB-BP466353SWN
CSB-MP466353SWN
CSB-EP466353SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 10 kDa chaperonin (groS) CSB-YP466354SWN
CSB-EP466354SWN
CSB-BP466354SWN
CSB-MP466354SWN
CSB-EP466354SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Uncharacterized protein YjeA (yjeA) CSB-YP466355SWN
CSB-EP466355SWN
CSB-BP466355SWN
CSB-MP466355SWN
CSB-EP466355SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Protein hfq (hfq) CSB-YP466356SWN
CSB-EP466356SWN
CSB-BP466356SWN
CSB-MP466356SWN
CSB-EP466356SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 3-keto-L-gulonate-6-phosphate decarboxylase UlaD (ulaD) CSB-YP466357SWN
CSB-EP466357SWN
CSB-BP466357SWN
CSB-MP466357SWN
CSB-EP466357SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Aspartate carbamoyltransferase (pyrB) CSB-YP466358SWN
CSB-EP466358SWN
CSB-BP466358SWN
CSB-MP466358SWN
CSB-EP466358SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP466359SWN
CSB-EP466359SWN
CSB-BP466359SWN
CSB-MP466359SWN
CSB-EP466359SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Glutamate--cysteine ligase (gshA), partial CSB-YP466360SWN
CSB-EP466360SWN
CSB-BP466360SWN
CSB-MP466360SWN
CSB-EP466360SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0386 protein yjhX (yjhX) CSB-YP466361SWN
CSB-EP466361SWN
CSB-BP466361SWN
CSB-MP466361SWN
CSB-EP466361SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Peptide chain release factor 3 (prfC), partial CSB-YP466362SWN
CSB-EP466362SWN
CSB-BP466362SWN
CSB-MP466362SWN
CSB-EP466362SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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