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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 7 Protein translocase subunit SecA (secA), partial CSB-YP465022AXI
CSB-EP465022AXI
CSB-BP465022AXI
CSB-MP465022AXI
CSB-EP465022AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP465023AXI
CSB-EP465023AXI
CSB-BP465023AXI
CSB-MP465023AXI
CSB-EP465023AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 UPF0756 membrane protein APP7_0388 (APP7_0388) CSB-YP465024AXI
CSB-EP465024AXI
CSB-BP465024AXI
CSB-MP465024AXI
CSB-EP465024AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Bifunctional protein GlmU (glmU) CSB-YP465025AXI
CSB-EP465025AXI
CSB-BP465025AXI
CSB-MP465025AXI
CSB-EP465025AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 30S ribosomal protein S9 (rpsI) CSB-YP465026AXI
CSB-EP465026AXI
CSB-BP465026AXI
CSB-MP465026AXI
CSB-EP465026AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosome-binding factor A (rbfA) CSB-YP465027AXI
CSB-EP465027AXI
CSB-BP465027AXI
CSB-MP465027AXI
CSB-EP465027AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Phosphoheptose isomerase (gmhA) CSB-YP465028AXI
CSB-EP465028AXI
CSB-BP465028AXI
CSB-MP465028AXI
CSB-EP465028AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 HTH-type transcriptional repressor PurR (purR) CSB-YP465029AXI
CSB-EP465029AXI
CSB-BP465029AXI
CSB-MP465029AXI
CSB-EP465029AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP465030AXI
CSB-EP465030AXI
CSB-BP465030AXI
CSB-MP465030AXI
CSB-EP465030AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 DNA gyrase inhibitor YacG (yacG) CSB-YP465031AXI
CSB-EP465031AXI
CSB-BP465031AXI
CSB-MP465031AXI
CSB-EP465031AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 10 kDa chaperonin (groS) CSB-YP465032AXI
CSB-EP465032AXI
CSB-BP465032AXI
CSB-MP465032AXI
CSB-EP465032AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 S-ribosylhomocysteine lyase (luxS) CSB-YP465033AXI
CSB-EP465033AXI
CSB-BP465033AXI
CSB-MP465033AXI
CSB-EP465033AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP465034AXI
CSB-EP465034AXI
CSB-BP465034AXI
CSB-MP465034AXI
CSB-EP465034AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP465035AXI
CSB-EP465035AXI
CSB-BP465035AXI
CSB-MP465035AXI
CSB-EP465035AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 30S ribosomal protein S7 (rpsG) CSB-YP465036AXI
CSB-EP465036AXI
CSB-BP465036AXI
CSB-MP465036AXI
CSB-EP465036AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 L-fucose mutarotase (fucU) CSB-YP465037AXI
CSB-EP465037AXI
CSB-BP465037AXI
CSB-MP465037AXI
CSB-EP465037AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At4g17616 (At4g17616), partial CSB-YP465038DOA
CSB-EP465038DOA
CSB-BP465038DOA
CSB-MP465038DOA
CSB-EP465038DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Diuretic hormone 41 (dh41) CSB-YP465039BTT
CSB-EP465039BTT
CSB-BP465039BTT
CSB-MP465039BTT
CSB-EP465039BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium knowlesi ATPase ASNA1 homolog (PKH_051870) CSB-YP465040EWU
CSB-EP465040EWU
CSB-BP465040EWU
CSB-MP465040EWU
CSB-EP465040EWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Spindle pole body component KRE28 (KRE28) CSB-YP465041SVP
CSB-EP465041SVP
CSB-BP465041SVP
CSB-MP465041SVP
CSB-EP465041SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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