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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 Protein ApaG (apaG) CSB-YP466823EOE
CSB-EP466823EOE
CSB-BP466823EOE
CSB-MP466823EOE
CSB-EP466823EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 2-isopropylmalate synthase (leuA) CSB-YP466824EOE
CSB-EP466824EOE
CSB-BP466824EOE
CSB-MP466824EOE
CSB-EP466824EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP466825EOE
CSB-EP466825EOE
CSB-BP466825EOE
CSB-MP466825EOE
CSB-EP466825EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Tryptophan synthase beta chain (trpB) CSB-YP466826EOE
CSB-EP466826EOE
CSB-BP466826EOE
CSB-MP466826EOE
CSB-EP466826EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Manganese transport protein MntH (mntH), partial CSB-YP466827EOE
CSB-EP466827EOE
CSB-BP466827EOE
CSB-MP466827EOE
CSB-EP466827EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Pyridoxine kinase (pdxK) CSB-YP466828EOE
CSB-EP466828EOE
CSB-BP466828EOE
CSB-MP466828EOE
CSB-EP466828EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 DnaA-homolog protein hda (hda) CSB-YP466829EOE
CSB-EP466829EOE
CSB-BP466829EOE
CSB-MP466829EOE
CSB-EP466829EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 NADH pyrophosphatase (nudC) CSB-YP466830EOE
CSB-EP466830EOE
CSB-BP466830EOE
CSB-MP466830EOE
CSB-EP466830EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Peptidase E (pepE) CSB-YP466831EOE
CSB-EP466831EOE
CSB-BP466831EOE
CSB-MP466831EOE
CSB-EP466831EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP466832EOE
CSB-EP466832EOE
CSB-BP466832EOE
CSB-MP466832EOE
CSB-EP466832EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD (+) reductase component CSB-YP466833EOE
CSB-EP466833EOE
CSB-BP466833EOE
CSB-MP466833EOE
CSB-EP466833EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP466834EOE
CSB-EP466834EOE
CSB-BP466834EOE
CSB-MP466834EOE
CSB-EP466834EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Chorismate--pyruvate lyase (ubiC) CSB-YP466835EOE
CSB-EP466835EOE
CSB-BP466835EOE
CSB-MP466835EOE
CSB-EP466835EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Esterase FrsA (frsA) CSB-YP466836EOE
CSB-EP466836EOE
CSB-BP466836EOE
CSB-MP466836EOE
CSB-EP466836EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Trans-aconitate 2-methyltransferase (tam) CSB-YP466837EOE
CSB-EP466837EOE
CSB-BP466837EOE
CSB-MP466837EOE
CSB-EP466837EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 tRNA (guanine-N (1)-)-methyltransferase CSB-YP466838EOE
CSB-EP466838EOE
CSB-BP466838EOE
CSB-MP466838EOE
CSB-EP466838EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein RnfH (rnfH) CSB-YP466839EOE
CSB-EP466839EOE
CSB-BP466839EOE
CSB-MP466839EOE
CSB-EP466839EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glutamate--cysteine ligase (gshA) CSB-YP466840EOE
CSB-EP466840EOE
CSB-BP466840EOE
CSB-MP466840EOE
CSB-EP466840EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Carbon storage regulator (csrA) CSB-YP466841EOE
CSB-EP466841EOE
CSB-BP466841EOE
CSB-MP466841EOE
CSB-EP466841EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Fumarate reductase subunit D (frdD), partial CSB-YP466842EOE1
CSB-EP466842EOE1
CSB-BP466842EOE1
CSB-MP466842EOE1
CSB-EP466842EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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