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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 7 Protein CyaY (cyaY) CSB-YP465002AXI
CSB-EP465002AXI
CSB-BP465002AXI
CSB-MP465002AXI
CSB-EP465002AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Probable transcriptional regulatory protein APP7_1210 (APP7_1210), partial CSB-YP465003AXI
CSB-EP465003AXI
CSB-BP465003AXI
CSB-MP465003AXI
CSB-EP465003AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 30S ribosomal protein S18 (rpsR) CSB-YP465004AXI
CSB-EP465004AXI
CSB-BP465004AXI
CSB-MP465004AXI
CSB-EP465004AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP465005AXI1
CSB-EP465005AXI1
CSB-BP465005AXI1
CSB-MP465005AXI1
CSB-EP465005AXI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Probable oxaloacetate decarboxylase gamma chain (oadG), partial CSB-YP465006AXI1
CSB-EP465006AXI1
CSB-BP465006AXI1
CSB-MP465006AXI1
CSB-EP465006AXI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP465007AXI
CSB-EP465007AXI
CSB-BP465007AXI
CSB-MP465007AXI
CSB-EP465007AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Hydroxylamine reductase (hcp), partial CSB-YP465008AXI
CSB-EP465008AXI
CSB-BP465008AXI
CSB-MP465008AXI
CSB-EP465008AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP465009AXI
CSB-EP465009AXI
CSB-BP465009AXI
CSB-MP465009AXI
CSB-EP465009AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Glucosamine-6-phosphate deaminase (nagB) CSB-YP465010AXI
CSB-EP465010AXI
CSB-BP465010AXI
CSB-MP465010AXI
CSB-EP465010AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 30S ribosomal protein S19 (rpsS) CSB-YP465011AXI
CSB-EP465011AXI
CSB-BP465011AXI
CSB-MP465011AXI
CSB-EP465011AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L15 (rplO) CSB-YP465012AXI
CSB-EP465012AXI
CSB-BP465012AXI
CSB-MP465012AXI
CSB-EP465012AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 tRNA (guanine-N (1)-)-methyltransferase CSB-YP465013AXI
CSB-EP465013AXI
CSB-BP465013AXI
CSB-MP465013AXI
CSB-EP465013AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Aspartate--ammonia ligase (asnA) CSB-YP465014AXI
CSB-EP465014AXI
CSB-BP465014AXI
CSB-MP465014AXI
CSB-EP465014AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP465015AXI
CSB-EP465015AXI
CSB-BP465015AXI
CSB-MP465015AXI
CSB-EP465015AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L21 (rplU) CSB-YP465016AXI
CSB-EP465016AXI
CSB-BP465016AXI
CSB-MP465016AXI
CSB-EP465016AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP465017AXI
CSB-EP465017AXI
CSB-BP465017AXI
CSB-MP465017AXI
CSB-EP465017AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP465018AXI
CSB-EP465018AXI
CSB-BP465018AXI
CSB-MP465018AXI
CSB-EP465018AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP465019AXI
CSB-EP465019AXI
CSB-BP465019AXI
CSB-MP465019AXI
CSB-EP465019AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP465020AXI
CSB-EP465020AXI
CSB-BP465020AXI
CSB-MP465020AXI
CSB-EP465020AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Biotin synthase (bioB) CSB-YP465021AXI
CSB-EP465021AXI
CSB-BP465021AXI
CSB-MP465021AXI
CSB-EP465021AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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