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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia cepacia 30S ribosomal protein S8 (rpsH) CSB-YP465323BXS
CSB-EP465323BXS
CSB-BP465323BXS
CSB-MP465323BXS
CSB-EP465323BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia 50S ribosomal protein L15 (rplO) CSB-YP465324BXS
CSB-EP465324BXS
CSB-BP465324BXS
CSB-MP465324BXS
CSB-EP465324BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia 30S ribosomal protein S4 (rpsD) CSB-YP465325BXS
CSB-EP465325BXS
CSB-BP465325BXS
CSB-MP465325BXS
CSB-EP465325BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP465326BXS
CSB-EP465326BXS
CSB-BP465326BXS
CSB-MP465326BXS
CSB-EP465326BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Potassium-transporting ATPase C chain (kdpC), partial CSB-YP465327BXS
CSB-EP465327BXS
CSB-BP465327BXS
CSB-MP465327BXS
CSB-EP465327BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Probable transcriptional regulatory protein BceJ2315_23480 (BceJ2315_23480) CSB-YP465328BXS
CSB-EP465328BXS
CSB-BP465328BXS
CSB-MP465328BXS
CSB-EP465328BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Glutamate 5-kinase (proB) CSB-YP465329BXS
CSB-EP465329BXS
CSB-BP465329BXS
CSB-MP465329BXS
CSB-EP465329BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP465330BXS
CSB-EP465330BXS
CSB-BP465330BXS
CSB-MP465330BXS
CSB-EP465330BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Sec-independent protein translocase protein TatA (tatA), partial CSB-YP465331BXS
CSB-EP465331BXS
CSB-BP465331BXS
CSB-MP465331BXS
CSB-EP465331BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia D-alanine--D-alanine ligase (ddl) CSB-YP465332BXS
CSB-EP465332BXS
CSB-BP465332BXS
CSB-MP465332BXS
CSB-EP465332BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP465333BXS
CSB-EP465333BXS
CSB-BP465333BXS
CSB-MP465333BXS
CSB-EP465333BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Putative membrane protein insertion efficiency factor (BceJ2315_04240) CSB-YP465334BXS
CSB-EP465334BXS
CSB-BP465334BXS
CSB-MP465334BXS
CSB-EP465334BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP465335BXS
CSB-EP465335BXS
CSB-BP465335BXS
CSB-MP465335BXS
CSB-EP465335BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia 7-cyano-7-deazaguanine synthase (queC) CSB-YP465336BXS
CSB-EP465336BXS
CSB-BP465336BXS
CSB-MP465336BXS
CSB-EP465336BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Homoserine O-acetyltransferase (metX) CSB-YP465337BXS
CSB-EP465337BXS
CSB-BP465337BXS
CSB-MP465337BXS
CSB-EP465337BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Ribose-5-phosphate isomerase A (rpiA) CSB-YP465338BXS
CSB-EP465338BXS
CSB-BP465338BXS
CSB-MP465338BXS
CSB-EP465338BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP465339BXS1
CSB-EP465339BXS1
CSB-BP465339BXS1
CSB-MP465339BXS1
CSB-EP465339BXS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia UPF0303 protein BceJ2315_15790 (BceJ2315_15790) CSB-YP465340BXS
CSB-EP465340BXS
CSB-BP465340BXS
CSB-MP465340BXS
CSB-EP465340BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP465341BXS
CSB-EP465341BXS
CSB-BP465341BXS
CSB-MP465341BXS
CSB-EP465341BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP465342BXS
CSB-EP465342BXS
CSB-BP465342BXS
CSB-MP465342BXS
CSB-EP465342BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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