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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Proteus mirabilis Secretion monitor (secM) CSB-YP465403EYZ
CSB-EP465403EYZ
CSB-BP465403EYZ
CSB-MP465403EYZ
CSB-EP465403EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Protein-export protein secB (secB) CSB-YP465404EYZ
CSB-EP465404EYZ
CSB-BP465404EYZ
CSB-MP465404EYZ
CSB-EP465404EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis ATP-dependent protease subunit HslV (hslV) CSB-YP465405EYZ
CSB-EP465405EYZ
CSB-BP465405EYZ
CSB-MP465405EYZ
CSB-EP465405EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP465406EYZ
CSB-EP465406EYZ
CSB-BP465406EYZ
CSB-MP465406EYZ
CSB-EP465406EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Protein sprT (sprT) CSB-YP465407EYZ
CSB-EP465407EYZ
CSB-BP465407EYZ
CSB-MP465407EYZ
CSB-EP465407EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis tRNA (uracil (54)-C (5))-methyltransferase CSB-YP465408EYZ
CSB-EP465408EYZ
CSB-BP465408EYZ
CSB-MP465408EYZ
CSB-EP465408EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L29 (rpmC) CSB-YP465409EYZ
CSB-EP465409EYZ
CSB-BP465409EYZ
CSB-MP465409EYZ
CSB-EP465409EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L6 (rplF) CSB-YP465410EYZ
CSB-EP465410EYZ
CSB-BP465410EYZ
CSB-MP465410EYZ
CSB-EP465410EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP465411EYZ
CSB-EP465411EYZ
CSB-BP465411EYZ
CSB-MP465411EYZ
CSB-EP465411EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA), partial CSB-YP465412EYZ
CSB-EP465412EYZ
CSB-BP465412EYZ
CSB-MP465412EYZ
CSB-EP465412EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis tRNA (Ile)-lysidine synthase CSB-YP465413EYZ
CSB-EP465413EYZ
CSB-BP465413EYZ
CSB-MP465413EYZ
CSB-EP465413EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis HTH-type transcriptional repressor NsrR (nsrR) CSB-YP465414EYZ
CSB-EP465414EYZ
CSB-BP465414EYZ
CSB-MP465414EYZ
CSB-EP465414EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Arginine repressor (argR) CSB-YP465415EYZ
CSB-EP465415EYZ
CSB-BP465415EYZ
CSB-MP465415EYZ
CSB-EP465415EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L27 (rpmA) CSB-YP465416EYZ
CSB-EP465416EYZ
CSB-BP465416EYZ
CSB-MP465416EYZ
CSB-EP465416EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ribosome-binding factor A (rbfA) CSB-YP465417EYZ
CSB-EP465417EYZ
CSB-BP465417EYZ
CSB-MP465417EYZ
CSB-EP465417EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Amino-acid acetyltransferase (argA) CSB-YP465418EYZ
CSB-EP465418EYZ
CSB-BP465418EYZ
CSB-MP465418EYZ
CSB-EP465418EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP465419EYZ
CSB-EP465419EYZ
CSB-BP465419EYZ
CSB-MP465419EYZ
CSB-EP465419EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis UPF0246 protein PMI0005 (PMI0005) CSB-YP465420EYZ
CSB-EP465420EYZ
CSB-BP465420EYZ
CSB-MP465420EYZ
CSB-EP465420EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Spindle and kinetochore-associated protein 1 homolog CSB-YP465421ZAX
CSB-EP465421ZAX
CSB-BP465421ZAX
CSB-MP465421ZAX
CSB-EP465421ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Male-specific protein scotti (soti) CSB-YP465422DMB
CSB-EP465422DMB
CSB-BP465422DMB
CSB-MP465422DMB
CSB-EP465422DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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