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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dictyoglomus thermophilum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP466723DKI
CSB-EP466723DKI
CSB-BP466723DKI
CSB-MP466723DKI
CSB-EP466723DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 30S ribosomal protein S2 (rpsB) CSB-YP466724DKI
CSB-EP466724DKI
CSB-BP466724DKI
CSB-MP466724DKI
CSB-EP466724DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP466725DKI
CSB-EP466725DKI
CSB-BP466725DKI
CSB-MP466725DKI
CSB-EP466725DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP466726DKI
CSB-EP466726DKI
CSB-BP466726DKI
CSB-MP466726DKI
CSB-EP466726DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L7/L12 (rplL) CSB-YP466727DKI
CSB-EP466727DKI
CSB-BP466727DKI
CSB-MP466727DKI
CSB-EP466727DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP466728DKI
CSB-EP466728DKI
CSB-BP466728DKI
CSB-MP466728DKI
CSB-EP466728DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP466729DKI
CSB-EP466729DKI
CSB-BP466729DKI
CSB-MP466729DKI
CSB-EP466729DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Phosphopentomutase (deoB) CSB-YP466730DKI
CSB-EP466730DKI
CSB-BP466730DKI
CSB-MP466730DKI
CSB-EP466730DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum 50S ribosomal protein L28 (rpmB) CSB-YP466731DKI
CSB-EP466731DKI
CSB-BP466731DKI
CSB-MP466731DKI
CSB-EP466731DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum UPF0102 protein DICTH_1420 (DICTH_1420) CSB-YP466732DKI
CSB-EP466732DKI
CSB-BP466732DKI
CSB-MP466732DKI
CSB-EP466732DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP466733DKI
CSB-EP466733DKI
CSB-BP466733DKI
CSB-MP466733DKI
CSB-EP466733DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP466734TMY
CSB-EP466734TMY
CSB-BP466734TMY
CSB-MP466734TMY
CSB-EP466734TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP466735TMY
CSB-EP466735TMY
CSB-BP466735TMY
CSB-MP466735TMY
CSB-EP466735TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 30S ribosomal protein S4 (rpsD) CSB-YP466736TMY
CSB-EP466736TMY
CSB-BP466736TMY
CSB-MP466736TMY
CSB-EP466736TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 30S ribosomal protein S14 type Z (rpsZ) CSB-YP466737TMY
CSB-EP466737TMY
CSB-BP466737TMY
CSB-MP466737TMY
CSB-EP466737TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Chromosomal replication initiator protein DnaA (dnaA) CSB-YP466738TMY
CSB-EP466738TMY
CSB-BP466738TMY
CSB-MP466738TMY
CSB-EP466738TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 3-dehydroquinate dehydratase (aroD) CSB-YP466739TMY
CSB-EP466739TMY
CSB-BP466739TMY
CSB-MP466739TMY
CSB-EP466739TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii N utilization substance protein B homolog (nusB) CSB-YP466740TMY
CSB-EP466740TMY
CSB-BP466740TMY
CSB-MP466740TMY
CSB-EP466740TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Ribosome-binding factor A (rbfA) CSB-YP466741TMY
CSB-EP466741TMY
CSB-BP466741TMY
CSB-MP466741TMY
CSB-EP466741TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Undecaprenyl-diphosphatase (uppP), partial CSB-YP466742TMY
CSB-EP466742TMY
CSB-BP466742TMY
CSB-MP466742TMY
CSB-EP466742TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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