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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella newport NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP465703SWS
CSB-EP465703SWS
CSB-BP465703SWS
CSB-MP465703SWS
CSB-EP465703SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Ketol-acid reductoisomerase (ilvC) CSB-YP465704SWS
CSB-EP465704SWS
CSB-BP465704SWS
CSB-MP465704SWS
CSB-EP465704SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Protein CyaY (cyaY) CSB-YP465705SWS
CSB-EP465705SWS
CSB-BP465705SWS
CSB-MP465705SWS
CSB-EP465705SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Endonuclease 8 (nei) CSB-YP465706SWS
CSB-EP465706SWS
CSB-BP465706SWS
CSB-MP465706SWS
CSB-EP465706SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Formimidoylglutamase (hutG) CSB-YP465707SWS
CSB-EP465707SWS
CSB-BP465707SWS
CSB-MP465707SWS
CSB-EP465707SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP465708SWS
CSB-EP465708SWS
CSB-BP465708SWS
CSB-MP465708SWS
CSB-EP465708SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Penicillin-insensitive murein endopeptidase (mepA) CSB-YP465709SWS
CSB-EP465709SWS
CSB-BP465709SWS
CSB-MP465709SWS
CSB-EP465709SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Rhamnulose-1-phosphate aldolase (rhaD) CSB-YP465710SWS
CSB-EP465710SWS
CSB-BP465710SWS
CSB-MP465710SWS
CSB-EP465710SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0194 membrane protein ybhG (ybhG) CSB-YP465711SWS
CSB-EP465711SWS
CSB-BP465711SWS
CSB-MP465711SWS
CSB-EP465711SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Putative transport protein YbjL (ybjL), partial CSB-YP465712SWS
CSB-EP465712SWS
CSB-BP465712SWS
CSB-MP465712SWS
CSB-EP465712SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport DnaA-homolog protein hda (hda) CSB-YP465713SWS
CSB-EP465713SWS
CSB-BP465713SWS
CSB-MP465713SWS
CSB-EP465713SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Cytoskeleton protein rodZ (rodZ), partial CSB-YP465714SWS
CSB-EP465714SWS
CSB-BP465714SWS
CSB-MP465714SWS
CSB-EP465714SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP465715SWS
CSB-EP465715SWS
CSB-BP465715SWS
CSB-MP465715SWS
CSB-EP465715SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Acetylornithine deacetylase (argE) CSB-YP465716SWS
CSB-EP465716SWS
CSB-BP465716SWS
CSB-MP465716SWS
CSB-EP465716SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Phosphomethylpyrimidine synthase (thiC), partial CSB-YP465717SWS
CSB-EP465717SWS
CSB-BP465717SWS
CSB-MP465717SWS
CSB-EP465717SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Homoserine O-succinyltransferase (metA) CSB-YP465718SWS
CSB-EP465718SWS
CSB-BP465718SWS
CSB-MP465718SWS
CSB-EP465718SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP465719SWS
CSB-EP465719SWS
CSB-BP465719SWS
CSB-MP465719SWS
CSB-EP465719SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Elongation factor 4 (lepA), partial CSB-YP465720SWS
CSB-EP465720SWS
CSB-BP465720SWS
CSB-MP465720SWS
CSB-EP465720SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Chorismate--pyruvate lyase (ubiC) CSB-YP465721SWS
CSB-EP465721SWS
CSB-BP465721SWS
CSB-MP465721SWS
CSB-EP465721SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport LexA repressor (lexA) CSB-YP465722SWS
CSB-EP465722SWS
CSB-BP465722SWS
CSB-MP465722SWS
CSB-EP465722SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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