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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella newport tRNA (mo5U34)-methyltransferase CSB-YP465683SWS
CSB-EP465683SWS
CSB-BP465683SWS
CSB-MP465683SWS
CSB-EP465683SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Shikimate kinase 1 (aroK) CSB-YP465684SWS
CSB-EP465684SWS
CSB-BP465684SWS
CSB-MP465684SWS
CSB-EP465684SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport HTH-type transcriptional regulator malT (malT), partial CSB-YP465685SWS
CSB-EP465685SWS
CSB-BP465685SWS
CSB-MP465685SWS
CSB-EP465685SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Glycogen synthase (glgA) CSB-YP465686SWS
CSB-EP465686SWS
CSB-BP465686SWS
CSB-MP465686SWS
CSB-EP465686SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Protein mtfA (mtfA) CSB-YP465687SWS
CSB-EP465687SWS
CSB-BP465687SWS
CSB-MP465687SWS
CSB-EP465687SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP465688SWS
CSB-EP465688SWS
CSB-BP465688SWS
CSB-MP465688SWS
CSB-EP465688SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport N utilization substance protein B homolog (nusB) CSB-YP465689SWS
CSB-EP465689SWS
CSB-BP465689SWS
CSB-MP465689SWS
CSB-EP465689SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Trigger factor (tig) CSB-YP465690SWS
CSB-EP465690SWS
CSB-BP465690SWS
CSB-MP465690SWS
CSB-EP465690SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 50S ribosomal protein L31 type B (rpmE2) CSB-YP465691SWS
CSB-EP465691SWS
CSB-BP465691SWS
CSB-MP465691SWS
CSB-EP465691SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Acetyl esterase (aes) CSB-YP465692SWS
CSB-EP465692SWS
CSB-BP465692SWS
CSB-MP465692SWS
CSB-EP465692SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport ATP phosphoribosyltransferase (hisG) CSB-YP465693SWS
CSB-EP465693SWS
CSB-BP465693SWS
CSB-MP465693SWS
CSB-EP465693SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP465694SWS
CSB-EP465694SWS
CSB-BP465694SWS
CSB-MP465694SWS
CSB-EP465694SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP465695SWS
CSB-EP465695SWS
CSB-BP465695SWS
CSB-MP465695SWS
CSB-EP465695SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport tRNA 2-selenouridine synthase (selU) CSB-YP465696SWS
CSB-EP465696SWS
CSB-BP465696SWS
CSB-MP465696SWS
CSB-EP465696SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Multidrug resistance protein MdtC (mdtC), partial CSB-YP465697SWS
CSB-EP465697SWS
CSB-BP465697SWS
CSB-MP465697SWS
CSB-EP465697SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Protein viaA (viaA) CSB-YP465698SWS
CSB-EP465698SWS
CSB-BP465698SWS
CSB-MP465698SWS
CSB-EP465698SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport D-ribose pyranase (rbsD) CSB-YP465699SWS
CSB-EP465699SWS
CSB-BP465699SWS
CSB-MP465699SWS
CSB-EP465699SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP465700SWS
CSB-EP465700SWS
CSB-BP465700SWS
CSB-MP465700SWS
CSB-EP465700SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Periplasmic nitrate reductase (napA), partial CSB-YP465701SWS
CSB-EP465701SWS
CSB-BP465701SWS
CSB-MP465701SWS
CSB-EP465701SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport o-succinylbenzoate synthase (menC) CSB-YP465702SWS
CSB-EP465702SWS
CSB-BP465702SWS
CSB-MP465702SWS
CSB-EP465702SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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