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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acidithiobacillus ferrooxidans tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP467743AWH
CSB-EP467743AWH
CSB-BP467743AWH
CSB-MP467743AWH
CSB-EP467743AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP467744AWH
CSB-EP467744AWH
CSB-BP467744AWH
CSB-MP467744AWH
CSB-EP467744AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Elongation factor P (efp) CSB-YP467745AWH
CSB-EP467745AWH
CSB-BP467745AWH
CSB-MP467745AWH
CSB-EP467745AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Protein RecA (recA) CSB-YP467746AWH
CSB-EP467746AWH
CSB-BP467746AWH
CSB-MP467746AWH
CSB-EP467746AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP467747AWH
CSB-EP467747AWH
CSB-BP467747AWH
CSB-MP467747AWH
CSB-EP467747AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L28 (rpmB) CSB-YP467748AWH
CSB-EP467748AWH
CSB-BP467748AWH
CSB-MP467748AWH
CSB-EP467748AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Alanine racemase (alr) CSB-YP467749AWH
CSB-EP467749AWH
CSB-BP467749AWH
CSB-MP467749AWH
CSB-EP467749AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Elongation factor 4 (lepA), partial CSB-YP467750AWH
CSB-EP467750AWH
CSB-BP467750AWH
CSB-MP467750AWH
CSB-EP467750AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Protein CrcB homolog (crcB), partial CSB-YP467751AWH1
CSB-EP467751AWH1
CSB-BP467751AWH1
CSB-MP467751AWH1
CSB-EP467751AWH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Probable Fe (2+)-trafficking protein CSB-YP467752AWH
CSB-EP467752AWH
CSB-BP467752AWH
CSB-MP467752AWH
CSB-EP467752AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP467753AWH
CSB-EP467753AWH
CSB-BP467753AWH
CSB-MP467753AWH
CSB-EP467753AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Chaperone protein htpG (htpG), partial CSB-YP467754AWH
CSB-EP467754AWH
CSB-BP467754AWH
CSB-MP467754AWH
CSB-EP467754AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Indole-3-glycerol phosphate synthase (trpC) CSB-YP467755AWH
CSB-EP467755AWH
CSB-BP467755AWH
CSB-MP467755AWH
CSB-EP467755AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Bifunctional protein FolD (folD) CSB-YP467756AWH
CSB-EP467756AWH
CSB-BP467756AWH
CSB-MP467756AWH
CSB-EP467756AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0178 protein BCAH820_3075 (BCAH820_3075) CSB-YP467757BQM
CSB-EP467757BQM
CSB-BP467757BQM
CSB-MP467757BQM
CSB-EP467757BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0349 protein BCAH820_5046 (BCAH820_5046) CSB-YP467758BQM
CSB-EP467758BQM
CSB-BP467758BQM
CSB-MP467758BQM
CSB-EP467758BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP467759BQM
CSB-EP467759BQM
CSB-BP467759BQM
CSB-MP467759BQM
CSB-EP467759BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methionine--tRNA ligase (metG), partial CSB-YP467760BQM
CSB-EP467760BQM
CSB-BP467760BQM
CSB-MP467760BQM
CSB-EP467760BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Tryptophan synthase alpha chain (trpA) CSB-YP467761BQM
CSB-EP467761BQM
CSB-BP467761BQM
CSB-MP467761BQM
CSB-EP467761BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus SsrA-binding protein (smpB) CSB-YP467762BQM
CSB-EP467762BQM
CSB-BP467762BQM
CSB-MP467762BQM
CSB-EP467762BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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