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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermodesulfovibrio yellowstonii Protein translocase subunit SecF (secF), partial CSB-YP466743TMY1
CSB-EP466743TMY1
CSB-BP466743TMY1
CSB-MP466743TMY1
CSB-EP466743TMY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Bifunctional NAD (P)H-hydrate repair enzyme Nnr, partial CSB-YP466744TMY
CSB-EP466744TMY
CSB-BP466744TMY
CSB-MP466744TMY
CSB-EP466744TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP466745TMY
CSB-EP466745TMY
CSB-BP466745TMY
CSB-MP466745TMY
CSB-EP466745TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii UPF0296 protein THEYE_A0405 (THEYE_A0405) CSB-YP466746TMY
CSB-EP466746TMY
CSB-BP466746TMY
CSB-MP466746TMY
CSB-EP466746TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Thiazole synthase (thiG) CSB-YP466747TMY
CSB-EP466747TMY
CSB-BP466747TMY
CSB-MP466747TMY
CSB-EP466747TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP466748TMY
CSB-EP466748TMY
CSB-BP466748TMY
CSB-MP466748TMY
CSB-EP466748TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 60 kDa chaperonin (groL), partial CSB-YP466749TMY
CSB-EP466749TMY
CSB-BP466749TMY
CSB-MP466749TMY
CSB-EP466749TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Phosphoheptose isomerase (gmhA) CSB-YP466750TMY
CSB-EP466750TMY
CSB-BP466750TMY
CSB-MP466750TMY
CSB-EP466750TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii UvrABC system protein B (uvrB), partial CSB-YP466751TMY
CSB-EP466751TMY
CSB-BP466751TMY
CSB-MP466751TMY
CSB-EP466751TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Biotin synthase (bioB) CSB-YP466752TMY
CSB-EP466752TMY
CSB-BP466752TMY
CSB-MP466752TMY
CSB-EP466752TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP466753TMY
CSB-EP466753TMY
CSB-BP466753TMY
CSB-MP466753TMY
CSB-EP466753TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Protease HtpX homolog (htpX), partial CSB-YP466754TMY1
CSB-EP466754TMY1
CSB-BP466754TMY1
CSB-MP466754TMY1
CSB-EP466754TMY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP466755TMY
CSB-EP466755TMY
CSB-BP466755TMY
CSB-MP466755TMY
CSB-EP466755TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Cobyric acid synthase (cobQ) CSB-YP466756TMY
CSB-EP466756TMY
CSB-BP466756TMY
CSB-MP466756TMY
CSB-EP466756TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP466757EOE
CSB-EP466757EOE
CSB-BP466757EOE
CSB-MP466757EOE
CSB-EP466757EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Citrate lyase acyl carrier protein (citD) CSB-YP466758EOE
CSB-EP466758EOE
CSB-BP466758EOE
CSB-MP466758EOE
CSB-EP466758EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Vitamin B12 import ATP-binding protein BtuD (btuD) CSB-YP466759EOE
CSB-EP466759EOE
CSB-BP466759EOE
CSB-MP466759EOE
CSB-EP466759EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Threonine--tRNA ligase (thrS) CSB-YP466760EOE
CSB-EP466760EOE
CSB-BP466760EOE
CSB-MP466760EOE
CSB-EP466760EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein ves (ves) CSB-YP466761EOE
CSB-EP466761EOE
CSB-BP466761EOE
CSB-MP466761EOE
CSB-EP466761EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Succinylornithine transaminase (astC) CSB-YP466762EOE
CSB-EP466762EOE
CSB-BP466762EOE
CSB-MP466762EOE
CSB-EP466762EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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