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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus 50S ribosomal protein L13 (rplM) CSB-YP467523BQO
CSB-EP467523BQO
CSB-BP467523BQO
CSB-MP467523BQO
CSB-EP467523BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP467524BQL
CSB-EP467524BQL
CSB-BP467524BQL
CSB-MP467524BQL
CSB-EP467524BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP467525BQL
CSB-EP467525BQL
CSB-BP467525BQL
CSB-MP467525BQL
CSB-EP467525BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP467526BQL
CSB-EP467526BQL
CSB-BP467526BQL
CSB-MP467526BQL
CSB-EP467526BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Hut operon positive regulatory protein (hutP) CSB-YP467527BQL
CSB-EP467527BQL
CSB-BP467527BQL
CSB-MP467527BQL
CSB-EP467527BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP467528BQL
CSB-EP467528BQL
CSB-BP467528BQL
CSB-MP467528BQL
CSB-EP467528BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP467529BQL
CSB-EP467529BQL
CSB-BP467529BQL
CSB-MP467529BQL
CSB-EP467529BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA mismatch repair protein mutL (mutL), partial CSB-YP467530BQL
CSB-EP467530BQL
CSB-BP467530BQL
CSB-MP467530BQL
CSB-EP467530BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosome-binding factor A (rbfA) CSB-YP467531BQL
CSB-EP467531BQL
CSB-BP467531BQL
CSB-MP467531BQL
CSB-EP467531BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP467532BQL
CSB-EP467532BQL
CSB-BP467532BQL
CSB-MP467532BQL
CSB-EP467532BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosome maturation factor RimM (rimM) CSB-YP467533BQL
CSB-EP467533BQL
CSB-BP467533BQL
CSB-MP467533BQL
CSB-EP467533BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Transcription factor fapR (fapR) CSB-YP467534BQL
CSB-EP467534BQL
CSB-BP467534BQL
CSB-MP467534BQL
CSB-EP467534BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit CSB-YP467535BQL
CSB-EP467535BQL
CSB-BP467535BQL
CSB-MP467535BQL
CSB-EP467535BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Isoleucine--tRNA ligase (ileS), partial CSB-YP467536BQL
CSB-EP467536BQL
CSB-BP467536BQL
CSB-MP467536BQL
CSB-EP467536BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Uncharacterized N-acetyltransferase BCAH187_A3974 (BCAH187_A3974) CSB-YP467537BQL
CSB-EP467537BQL
CSB-BP467537BQL
CSB-MP467537BQL
CSB-EP467537BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0358 protein BCAH187_A4068 (BCAH187_A4068) CSB-YP467538BQL
CSB-EP467538BQL
CSB-BP467538BQL
CSB-MP467538BQL
CSB-EP467538BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein-glutamine gamma-glutamyltransferase (tgl) CSB-YP467539BQL
CSB-EP467539BQL
CSB-BP467539BQL
CSB-MP467539BQL
CSB-EP467539BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N-acetyldiaminopimelate deacetylase (BCAH187_A4100) CSB-YP467540BQL
CSB-EP467540BQL
CSB-BP467540BQL
CSB-MP467540BQL
CSB-EP467540BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP467541BQL
CSB-EP467541BQL
CSB-BP467541BQL
CSB-MP467541BQL
CSB-EP467541BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Anti-sigma F factor (spoIIAB) CSB-YP467542BQL
CSB-EP467542BQL
CSB-BP467542BQL
CSB-MP467542BQL
CSB-EP467542BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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