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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP467563BQL
CSB-EP467563BQL
CSB-BP467563BQL
CSB-MP467563BQL
CSB-EP467563BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP467564BQL
CSB-EP467564BQL
CSB-BP467564BQL
CSB-MP467564BQL
CSB-EP467564BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Septation ring formation regulator EzrA (ezrA), partial CSB-YP467565BQL
CSB-EP467565BQL
CSB-BP467565BQL
CSB-MP467565BQL
CSB-EP467565BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Leucine--tRNA ligase (leuS), partial CSB-YP467566BQL
CSB-EP467566BQL
CSB-BP467566BQL
CSB-MP467566BQL
CSB-EP467566BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP467567BQL
CSB-EP467567BQL
CSB-BP467567BQL
CSB-MP467567BQL
CSB-EP467567BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Thiamine-phosphate synthase (thiE) CSB-YP467568BQL
CSB-EP467568BQL
CSB-BP467568BQL
CSB-MP467568BQL
CSB-EP467568BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP467569BQL
CSB-EP467569BQL
CSB-BP467569BQL
CSB-MP467569BQL
CSB-EP467569BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0295 protein BCAH187_A0594 (BCAH187_A0594), partial CSB-YP467570BQL1
CSB-EP467570BQL1
CSB-BP467570BQL1
CSB-MP467570BQL1
CSB-EP467570BQL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0349 protein BCAH187_A5080 (BCAH187_A5080) CSB-YP467571BQL
CSB-EP467571BQL
CSB-BP467571BQL
CSB-MP467571BQL
CSB-EP467571BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Gamma-glutamyl phosphate reductase (proA) CSB-YP467572BQL
CSB-EP467572BQL
CSB-BP467572BQL
CSB-MP467572BQL
CSB-EP467572BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoglycerate kinase (pgk) CSB-YP467573BQL
CSB-EP467573BQL
CSB-BP467573BQL
CSB-MP467573BQL
CSB-EP467573BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Thiazole synthase (thiG) CSB-YP467574BQL
CSB-EP467574BQL
CSB-BP467574BQL
CSB-MP467574BQL
CSB-EP467574BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein ArsC (arsC) CSB-YP467575BQL
CSB-EP467575BQL
CSB-BP467575BQL
CSB-MP467575BQL
CSB-EP467575BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP467576BQL
CSB-EP467576BQL
CSB-BP467576BQL
CSB-MP467576BQL
CSB-EP467576BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0316 protein BCAH187_A3394 (BCAH187_A3394), partial CSB-YP467577BQL1
CSB-EP467577BQL1
CSB-BP467577BQL1
CSB-MP467577BQL1
CSB-EP467577BQL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0059 membrane protein BCAH187_A5502 (BCAH187_A5502), partial CSB-YP467578BQL1
CSB-EP467578BQL1
CSB-BP467578BQL1
CSB-MP467578BQL1
CSB-EP467578BQL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3'-5' exoribonuclease yhaM (yhaM) CSB-YP467579BQL
CSB-EP467579BQL
CSB-BP467579BQL
CSB-MP467579BQL
CSB-EP467579BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S6 (rpsF) CSB-YP467580BQL
CSB-EP467580BQL
CSB-BP467580BQL
CSB-MP467580BQL
CSB-EP467580BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0738 protein BCAH187_A1357 (BCAH187_A1357) CSB-YP467581BQL
CSB-EP467581BQL
CSB-BP467581BQL
CSB-MP467581BQL
CSB-EP467581BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Tryptophan synthase alpha chain (trpA) CSB-YP467582BQL
CSB-EP467582BQL
CSB-BP467582BQL
CSB-MP467582BQL
CSB-EP467582BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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