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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Aliivibrio salmonicida Autonomous glycyl radical cofactor (grcA) CSB-YP469323AZM
CSB-EP469323AZM
CSB-BP469323AZM
CSB-MP469323AZM
CSB-EP469323AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP469324AZM
CSB-EP469324AZM
CSB-BP469324AZM
CSB-MP469324AZM
CSB-EP469324AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Sugar fermentation stimulation protein homolog (sfsA) CSB-YP469325AZM
CSB-EP469325AZM
CSB-BP469325AZM
CSB-MP469325AZM
CSB-EP469325AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Protein translocase subunit SecA (secA), partial CSB-YP469326AZM
CSB-EP469326AZM
CSB-BP469326AZM
CSB-MP469326AZM
CSB-EP469326AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida UPF0102 protein VSAL_I2655 (VSAL_I2655) CSB-YP469327AZM
CSB-EP469327AZM
CSB-BP469327AZM
CSB-MP469327AZM
CSB-EP469327AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 2-isopropylmalate synthase (leuA), partial CSB-YP469328AZM
CSB-EP469328AZM
CSB-BP469328AZM
CSB-MP469328AZM
CSB-EP469328AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Sulfate adenylyltransferase subunit 1 (cysN) CSB-YP469329AZM
CSB-EP469329AZM
CSB-BP469329AZM
CSB-MP469329AZM
CSB-EP469329AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP469330AZM1
CSB-EP469330AZM1
CSB-BP469330AZM1
CSB-MP469330AZM1
CSB-EP469330AZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP469331AZM
CSB-EP469331AZM
CSB-BP469331AZM
CSB-MP469331AZM
CSB-EP469331AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 30S ribosomal protein S6 (rpsF) CSB-YP469332AZM
CSB-EP469332AZM
CSB-BP469332AZM
CSB-MP469332AZM
CSB-EP469332AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Fumarate reductase subunit D (frdD), partial CSB-YP469333AZM1
CSB-EP469333AZM1
CSB-BP469333AZM1
CSB-MP469333AZM1
CSB-EP469333AZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida tRNA (guanine-N (7)-)-methyltransferase CSB-YP469334AZM
CSB-EP469334AZM
CSB-BP469334AZM
CSB-MP469334AZM
CSB-EP469334AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Phosphoglycerate kinase (pgk) CSB-YP469335AZM
CSB-EP469335AZM
CSB-BP469335AZM
CSB-MP469335AZM
CSB-EP469335AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Integration host factor subunit alpha (ihfA) CSB-YP469336AZM
CSB-EP469336AZM
CSB-BP469336AZM
CSB-MP469336AZM
CSB-EP469336AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP469337AZM
CSB-EP469337AZM
CSB-BP469337AZM
CSB-MP469337AZM
CSB-EP469337AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP469338AZM
CSB-EP469338AZM
CSB-BP469338AZM
CSB-MP469338AZM
CSB-EP469338AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Ribosome-binding factor A (rbfA) CSB-YP469339AZM
CSB-EP469339AZM
CSB-BP469339AZM
CSB-MP469339AZM
CSB-EP469339AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Phosphopentomutase (deoB) CSB-YP469340AZM
CSB-EP469340AZM
CSB-BP469340AZM
CSB-MP469340AZM
CSB-EP469340AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP469341AZM
CSB-EP469341AZM
CSB-BP469341AZM
CSB-MP469341AZM
CSB-EP469341AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida LexA repressor (lexA) CSB-YP469342AZM
CSB-EP469342AZM
CSB-BP469342AZM
CSB-MP469342AZM
CSB-EP469342AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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