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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acidithiobacillus ferrooxidans Peptidyl-tRNA hydrolase (pth) CSB-YP470783AWI
CSB-EP470783AWI
CSB-BP470783AWI
CSB-MP470783AWI
CSB-EP470783AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP470784AWI
CSB-EP470784AWI
CSB-BP470784AWI
CSB-MP470784AWI
CSB-EP470784AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP470785AWI
CSB-EP470785AWI
CSB-BP470785AWI
CSB-MP470785AWI
CSB-EP470785AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP470786AWI
CSB-EP470786AWI
CSB-BP470786AWI
CSB-MP470786AWI
CSB-EP470786AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP470787AWI
CSB-EP470787AWI
CSB-BP470787AWI
CSB-MP470787AWI
CSB-EP470787AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP470788AWI
CSB-EP470788AWI
CSB-BP470788AWI
CSB-MP470788AWI
CSB-EP470788AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP470789AWI
CSB-EP470789AWI
CSB-BP470789AWI
CSB-MP470789AWI
CSB-EP470789AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L24 (rplX) CSB-YP470790AWI
CSB-EP470790AWI
CSB-BP470790AWI
CSB-MP470790AWI
CSB-EP470790AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L15 (rplO) CSB-YP470791AWI
CSB-EP470791AWI
CSB-BP470791AWI
CSB-MP470791AWI
CSB-EP470791AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Serine--tRNA ligase (serS) CSB-YP470792AWI
CSB-EP470792AWI
CSB-BP470792AWI
CSB-MP470792AWI
CSB-EP470792AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L20 (rplT) CSB-YP470793AWI
CSB-EP470793AWI
CSB-BP470793AWI
CSB-MP470793AWI
CSB-EP470793AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Deoxycytidine triphosphate deaminase (dcd) CSB-YP470794AWI
CSB-EP470794AWI
CSB-BP470794AWI
CSB-MP470794AWI
CSB-EP470794AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP470795AWI
CSB-EP470795AWI
CSB-BP470795AWI
CSB-MP470795AWI
CSB-EP470795AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Probable intracellular septation protein A (Lferr_0816), partial CSB-YP470796AWI1
CSB-EP470796AWI1
CSB-BP470796AWI1
CSB-MP470796AWI1
CSB-EP470796AWI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Ketol-acid reductoisomerase (ilvC) CSB-YP470797AWI
CSB-EP470797AWI
CSB-BP470797AWI
CSB-MP470797AWI
CSB-EP470797AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP470798AWI
CSB-EP470798AWI
CSB-BP470798AWI
CSB-MP470798AWI
CSB-EP470798AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Sugar fermentation stimulation protein homolog (sfsA) CSB-YP470799AWI
CSB-EP470799AWI
CSB-BP470799AWI
CSB-MP470799AWI
CSB-EP470799AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans UPF0042 nucleotide-binding protein Lferr_2629 (Lferr_2629) CSB-YP470800AWI
CSB-EP470800AWI
CSB-BP470800AWI
CSB-MP470800AWI
CSB-EP470800AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP470801AWI
CSB-EP470801AWI
CSB-BP470801AWI
CSB-MP470801AWI
CSB-EP470801AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP470802AWI
CSB-EP470802AWI
CSB-BP470802AWI
CSB-MP470802AWI
CSB-EP470802AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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