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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP472185BQO
CSB-EP472185BQO
CSB-BP472185BQO
CSB-MP472185BQO
CSB-EP472185BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0756 membrane protein BCB4264_A4705 (BCB4264_A4705), partial CSB-YP472186BQO1
CSB-EP472186BQO1
CSB-BP472186BQO1
CSB-MP472186BQO1
CSB-EP472186BQO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L31 type B (rpmE2) CSB-YP472187BQO
CSB-EP472187BQO
CSB-BP472187BQO
CSB-MP472187BQO
CSB-EP472187BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycerol kinase (glpK) CSB-YP472188BQO
CSB-EP472188BQO
CSB-BP472188BQO
CSB-MP472188BQO
CSB-EP472188BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Antiholin-like protein LrgA (lrgA), partial CSB-YP472189BQO1
CSB-EP472189BQO1
CSB-BP472189BQO1
CSB-MP472189BQO1
CSB-EP472189BQO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0738 protein BCB4264_A1252 (BCB4264_A1252) CSB-YP472190BQO
CSB-EP472190BQO
CSB-BP472190BQO
CSB-MP472190BQO
CSB-EP472190BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Tryptophan synthase beta chain (trpB) CSB-YP472191BQO
CSB-EP472191BQO
CSB-BP472191BQO
CSB-MP472191BQO
CSB-EP472191BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Lactate utilization protein B (lutB) CSB-YP472192BQO
CSB-EP472192BQO
CSB-BP472192BQO
CSB-MP472192BQO
CSB-EP472192BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0180 protein BCB4264_A1446 (BCB4264_A1446) CSB-YP472193BQO
CSB-EP472193BQO
CSB-BP472193BQO
CSB-MP472193BQO
CSB-EP472193BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP phosphoribosyltransferase (hisG) CSB-YP472194BQO
CSB-EP472194BQO
CSB-BP472194BQO
CSB-MP472194BQO
CSB-EP472194BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP472195BQO
CSB-EP472195BQO
CSB-BP472195BQO
CSB-MP472195BQO
CSB-EP472195BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP472196BQO
CSB-EP472196BQO
CSB-BP472196BQO
CSB-MP472196BQO
CSB-EP472196BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus CCA-adding enzyme (cca) CSB-YP472197BQO
CSB-EP472197BQO
CSB-BP472197BQO
CSB-MP472197BQO
CSB-EP472197BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Xanthine phosphoribosyltransferase (xpt) CSB-YP472198BQO
CSB-EP472198BQO
CSB-BP472198BQO
CSB-MP472198BQO
CSB-EP472198BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP472199BQO
CSB-EP472199BQO
CSB-BP472199BQO
CSB-MP472199BQO
CSB-EP472199BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0176 protein BCB4264_A1875 (BCB4264_A1875) CSB-YP472200BQO
CSB-EP472200BQO
CSB-BP472200BQO
CSB-MP472200BQO
CSB-EP472200BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA integrity scanning protein DisA (disA) CSB-YP472201BQO
CSB-EP472201BQO
CSB-BP472201BQO
CSB-MP472201BQO
CSB-EP472201BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP472202BQO
CSB-EP472202BQO
CSB-BP472202BQO
CSB-MP472202BQO
CSB-EP472202BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L2 (rplB) CSB-YP472203BQO
CSB-EP472203BQO
CSB-BP472203BQO
CSB-MP472203BQO
CSB-EP472203BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L18 (rplR) CSB-YP472204BQO
CSB-EP472204BQO
CSB-BP472204BQO
CSB-MP472204BQO
CSB-EP472204BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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