Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella agona Aspartate--ammonia ligase (asnA) CSB-YP470823SWK
CSB-EP470823SWK
CSB-BP470823SWK
CSB-MP470823SWK
CSB-EP470823SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Ketol-acid reductoisomerase (ilvC) CSB-YP470824SWK
CSB-EP470824SWK
CSB-BP470824SWK
CSB-MP470824SWK
CSB-EP470824SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Putative ECA polymerase (wzyE), partial CSB-YP470825SWK
CSB-EP470825SWK
CSB-BP470825SWK
CSB-MP470825SWK
CSB-EP470825SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP470826SWK
CSB-EP470826SWK
CSB-BP470826SWK
CSB-MP470826SWK
CSB-EP470826SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Potassium-transporting ATPase C chain (kdpC), partial CSB-YP470827SWK1
CSB-EP470827SWK1
CSB-BP470827SWK1
CSB-MP470827SWK1
CSB-EP470827SWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP470828SWK
CSB-EP470828SWK
CSB-BP470828SWK
CSB-MP470828SWK
CSB-EP470828SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Chorismate synthase (aroC) CSB-YP470829SWK
CSB-EP470829SWK
CSB-BP470829SWK
CSB-MP470829SWK
CSB-EP470829SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Probable GTP-binding protein EngB (engB) CSB-YP470830SWK
CSB-EP470830SWK
CSB-BP470830SWK
CSB-MP470830SWK
CSB-EP470830SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Quinolinate synthase A (nadA) CSB-YP470831SWK
CSB-EP470831SWK
CSB-BP470831SWK
CSB-MP470831SWK
CSB-EP470831SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Formimidoylglutamase (hutG) CSB-YP470832SWK
CSB-EP470832SWK
CSB-BP470832SWK
CSB-MP470832SWK
CSB-EP470832SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona DNA protection during starvation protein (dps) CSB-YP470833SWK
CSB-EP470833SWK
CSB-BP470833SWK
CSB-MP470833SWK
CSB-EP470833SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Cell division protein ZipA homolog (zipA), partial CSB-YP470834SWK
CSB-EP470834SWK
CSB-BP470834SWK
CSB-MP470834SWK
CSB-EP470834SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona L-rhamnose mutarotase (rhaM) CSB-YP470835SWK
CSB-EP470835SWK
CSB-BP470835SWK
CSB-MP470835SWK
CSB-EP470835SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Glycerol kinase (glpK), partial CSB-YP470836SWK
CSB-EP470836SWK
CSB-BP470836SWK
CSB-MP470836SWK
CSB-EP470836SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Putative transport protein YbjL (ybjL), partial CSB-YP470837SWK
CSB-EP470837SWK
CSB-BP470837SWK
CSB-MP470837SWK
CSB-EP470837SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Serine--tRNA ligase (serS) CSB-YP470838SWK
CSB-EP470838SWK
CSB-BP470838SWK
CSB-MP470838SWK
CSB-EP470838SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP470839SWK
CSB-EP470839SWK
CSB-BP470839SWK
CSB-MP470839SWK
CSB-EP470839SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Histidine--tRNA ligase (hisS) CSB-YP470840SWK
CSB-EP470840SWK
CSB-BP470840SWK
CSB-MP470840SWK
CSB-EP470840SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Thiazole synthase (thiG) CSB-YP470841SWK
CSB-EP470841SWK
CSB-BP470841SWK
CSB-MP470841SWK
CSB-EP470841SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Homoserine O-succinyltransferase (metA) CSB-YP470842SWK
CSB-EP470842SWK
CSB-BP470842SWK
CSB-MP470842SWK
CSB-EP470842SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>