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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit CSB-YP478566BQO
CSB-EP478566BQO
CSB-BP478566BQO
CSB-MP478566BQO
CSB-EP478566BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cell division protein SepF (sepF) CSB-YP478567BQO
CSB-EP478567BQO
CSB-BP478567BQO
CSB-MP478567BQO
CSB-EP478567BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0747 protein BCB4264_A4020 (BCB4264_A4020), partial CSB-YP478568BQO
CSB-EP478568BQO
CSB-BP478568BQO
CSB-MP478568BQO
CSB-EP478568BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Formamidase (amiF) CSB-YP478569BQO
CSB-EP478569BQO
CSB-BP478569BQO
CSB-MP478569BQO
CSB-EP478569BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N-acetyldiaminopimelate deacetylase (BCB4264_A4083) CSB-YP478570BQO
CSB-EP478570BQO
CSB-BP478570BQO
CSB-MP478570BQO
CSB-EP478570BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable tRNA sulfurtransferase (thiI) CSB-YP478571BQO
CSB-EP478571BQO
CSB-BP478571BQO
CSB-MP478571BQO
CSB-EP478571BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Hydroxyethylthiazole kinase (thiM) CSB-YP478572BQO
CSB-EP478572BQO
CSB-BP478572BQO
CSB-MP478572BQO
CSB-EP478572BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0397 protein BCB4264_A2665 (BCB4264_A2665), partial CSB-YP478573BQO1
CSB-EP478573BQO1
CSB-BP478573BQO1
CSB-MP478573BQO1
CSB-EP478573BQO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0271 protein BCB4264_A3086 (BCB4264_A3086) CSB-YP478574BQO
CSB-EP478574BQO
CSB-BP478574BQO
CSB-MP478574BQO
CSB-EP478574BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP478575BQO
CSB-EP478575BQO
CSB-BP478575BQO
CSB-MP478575BQO
CSB-EP478575BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Segregation and condensation protein A (scpA) CSB-YP478576BQO
CSB-EP478576BQO
CSB-BP478576BQO
CSB-MP478576BQO
CSB-EP478576BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP478577BQO
CSB-EP478577BQO
CSB-BP478577BQO
CSB-MP478577BQO
CSB-EP478577BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Regulatory protein recX (recX) CSB-YP478578BQO
CSB-EP478578BQO
CSB-BP478578BQO
CSB-MP478578BQO
CSB-EP478578BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable transcriptional regulatory protein BCB4264_A0577 (BCB4264_A0577) CSB-YP478579BQO
CSB-EP478579BQO
CSB-BP478579BQO
CSB-MP478579BQO
CSB-EP478579BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP478580BQO
CSB-EP478580BQO
CSB-BP478580BQO
CSB-MP478580BQO
CSB-EP478580BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP478581BQO
CSB-EP478581BQO
CSB-BP478581BQO
CSB-MP478581BQO
CSB-EP478581BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP478582BQO
CSB-EP478582BQO
CSB-BP478582BQO
CSB-MP478582BQO
CSB-EP478582BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Transcriptional regulator MntR (mntR) CSB-YP478583BQO
CSB-EP478583BQO
CSB-BP478583BQO
CSB-MP478583BQO
CSB-EP478583BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0349 protein BCB4264_A5077 (BCB4264_A5077) CSB-YP478584BQO
CSB-EP478584BQO
CSB-BP478584BQO
CSB-MP478584BQO
CSB-EP478584BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Hut operon positive regulatory protein (hutP) CSB-YP478585BQO
CSB-EP478585BQO
CSB-BP478585BQO
CSB-MP478585BQO
CSB-EP478585BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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