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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Helicobacter pylori 30S ribosomal protein S19 (rpsS) CSB-YP478359HUY
CSB-EP478359HUY
CSB-BP478359HUY
CSB-MP478359HUY
CSB-EP478359HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Putative nickel-responsive regulator (HPP12_1301) CSB-YP478360HUY
CSB-EP478360HUY
CSB-BP478360HUY
CSB-MP478360HUY
CSB-EP478360HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Putative membrane protein insertion efficiency factor (HPP12_1426) CSB-YP478361HUY
CSB-EP478361HUY
CSB-BP478361HUY
CSB-MP478361HUY
CSB-EP478361HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Peptidyl-tRNA hydrolase (pth) CSB-YP478362HUY
CSB-EP478362HUY
CSB-BP478362HUY
CSB-MP478362HUY
CSB-EP478362HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Flagellar hook-basal body complex protein FliE (fliE) CSB-YP478363HUY
CSB-EP478363HUY
CSB-BP478363HUY
CSB-MP478363HUY
CSB-EP478363HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP478364HUY
CSB-EP478364HUY
CSB-BP478364HUY
CSB-MP478364HUY
CSB-EP478364HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori UPF0133 protein HPP12_0031 (HPP12_0031) CSB-YP478365HUY
CSB-EP478365HUY
CSB-BP478365HUY
CSB-MP478365HUY
CSB-EP478365HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Lipoprotein signal peptidase (lspA), partial CSB-YP478366HUY1
CSB-EP478366HUY1
CSB-BP478366HUY1
CSB-MP478366HUY1
CSB-EP478366HUY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori S-ribosylhomocysteine lyase (luxS) CSB-YP478367HUY
CSB-EP478367HUY
CSB-BP478367HUY
CSB-MP478367HUY
CSB-EP478367HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Enolase (eno) CSB-YP478368HUY
CSB-EP478368HUY
CSB-BP478368HUY
CSB-MP478368HUY
CSB-EP478368HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori UPF0114 protein HPP12_0190 (HPP12_0190), partial CSB-YP478369HUY1
CSB-EP478369HUY1
CSB-BP478369HUY1
CSB-MP478369HUY1
CSB-EP478369HUY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Vacuolar protein sorting/targeting protein 10 (vps10), partial CSB-YP478370SXT
CSB-EP478370SXT
CSB-BP478370SXT
CSB-MP478370SXT
CSB-EP478370SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus ATPase get3 (get3) CSB-YP478371SXT
CSB-EP478371SXT
CSB-BP478371SXT
CSB-MP478371SXT
CSB-EP478371SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Mitochondrial group I intron splicing factor dmr1 (dmr1), partial CSB-YP478372SXT
CSB-EP478372SXT
CSB-BP478372SXT
CSB-MP478372SXT
CSB-EP478372SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Probable zinc metalloprotease SJAG_03009 (SJAG_03009), partial CSB-YP478373SXT
CSB-EP478373SXT
CSB-BP478373SXT
CSB-MP478373SXT
CSB-EP478373SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus ASTRA-associated protein 1 (asa1) CSB-YP478374SXT
CSB-EP478374SXT
CSB-BP478374SXT
CSB-MP478374SXT
CSB-EP478374SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma floridae UPF0722 protein (BRAFLDRAFT_119565) CSB-YP478375BVY
CSB-EP478375BVY
CSB-BP478375BVY
CSB-MP478375BVY
CSB-EP478375BVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Tethering factor for nuclear proteasome sts1 (sts1) CSB-YP478376ETM
CSB-EP478376ETM
CSB-BP478376ETM
CSB-MP478376ETM
CSB-EP478376ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Glutamyl-tRNA (Gln) amidotransferase subunit B, mitochondrial, partial CSB-YP478377ETM
CSB-EP478377ETM
CSB-BP478377ETM
CSB-MP478377ETM
CSB-EP478377ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Probable zinc metalloprotease PMAA_024220 (PMAA_024220), partial CSB-YP478378ETM
CSB-EP478378ETM
CSB-BP478378ETM
CSB-MP478378ETM
CSB-EP478378ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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