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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus 50S ribosomal protein L2 (rplB) CSB-YP478806BQP
CSB-EP478806BQP
CSB-BP478806BQP
CSB-MP478806BQP
CSB-EP478806BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L14 (rplN) CSB-YP478807BQP
CSB-EP478807BQP
CSB-BP478807BQP
CSB-MP478807BQP
CSB-EP478807BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L18 (rplR) CSB-YP478808BQP
CSB-EP478808BQP
CSB-BP478808BQP
CSB-MP478808BQP
CSB-EP478808BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L15 (rplO) CSB-YP478809BQP
CSB-EP478809BQP
CSB-BP478809BQP
CSB-MP478809BQP
CSB-EP478809BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L13 (rplM) CSB-YP478810BQP
CSB-EP478810BQP
CSB-BP478810BQP
CSB-MP478810BQP
CSB-EP478810BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Metallothiol transferase fosB (fosB) CSB-YP478811BQP
CSB-EP478811BQP
CSB-BP478811BQP
CSB-MP478811BQP
CSB-EP478811BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative competence-damage inducible protein (cinA) CSB-YP478812BQP
CSB-EP478812BQP
CSB-BP478812BQP
CSB-MP478812BQP
CSB-EP478812BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S15 (rpsO) CSB-YP478813BQP
CSB-EP478813BQP
CSB-BP478813BQP
CSB-MP478813BQP
CSB-EP478813BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0122 protein BCG9842_B1298 (BCG9842_B1298) CSB-YP478814BQP
CSB-EP478814BQP
CSB-BP478814BQP
CSB-MP478814BQP
CSB-EP478814BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit CSB-YP478815BQP
CSB-EP478815BQP
CSB-BP478815BQP
CSB-MP478815BQP
CSB-EP478815BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Isoleucine--tRNA ligase (ileS), partial CSB-YP478816BQP
CSB-EP478816BQP
CSB-BP478816BQP
CSB-MP478816BQP
CSB-EP478816BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 60 kDa chaperonin (groL), partial CSB-YP478817BQP
CSB-EP478817BQP
CSB-BP478817BQP
CSB-MP478817BQP
CSB-EP478817BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP478818BQP
CSB-EP478818BQP
CSB-BP478818BQP
CSB-MP478818BQP
CSB-EP478818BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP478819BQP
CSB-EP478819BQP
CSB-BP478819BQP
CSB-MP478819BQP
CSB-EP478819BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L32 (rpmF) CSB-YP478820BQP
CSB-EP478820BQP
CSB-BP478820BQP
CSB-MP478820BQP
CSB-EP478820BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0223 protein BCG9842_B1176 (BCG9842_B1176) CSB-YP478821BQP
CSB-EP478821BQP
CSB-BP478821BQP
CSB-MP478821BQP
CSB-EP478821BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP478822BQP
CSB-EP478822BQP
CSB-BP478822BQP
CSB-MP478822BQP
CSB-EP478822BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Regulatory protein recX (recX) CSB-YP478823BQP
CSB-EP478823BQP
CSB-BP478823BQP
CSB-MP478823BQP
CSB-EP478823BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP478824BQP
CSB-EP478824BQP
CSB-BP478824BQP
CSB-MP478824BQP
CSB-EP478824BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Anti-sigma F factor (spoIIAB) CSB-YP478825BQP
CSB-EP478825BQP
CSB-BP478825BQP
CSB-MP478825BQP
CSB-EP478825BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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