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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella agona Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP479606SWK
CSB-EP479606SWK
CSB-BP479606SWK
CSB-MP479606SWK
CSB-EP479606SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Fatty acid metabolism regulator protein (fadR) CSB-YP479607SWK
CSB-EP479607SWK
CSB-BP479607SWK
CSB-MP479607SWK
CSB-EP479607SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP479608SWK
CSB-EP479608SWK
CSB-BP479608SWK
CSB-MP479608SWK
CSB-EP479608SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Enolase (eno) CSB-YP479609SWK
CSB-EP479609SWK
CSB-BP479609SWK
CSB-MP479609SWK
CSB-EP479609SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP479610SWK1
CSB-EP479610SWK1
CSB-BP479610SWK1
CSB-MP479610SWK1
CSB-EP479610SWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Primosomal protein 1 (dnaT) CSB-YP479611SWK
CSB-EP479611SWK
CSB-BP479611SWK
CSB-MP479611SWK
CSB-EP479611SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP479612SWK
CSB-EP479612SWK
CSB-BP479612SWK
CSB-MP479612SWK
CSB-EP479612SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP479613SWK
CSB-EP479613SWK
CSB-BP479613SWK
CSB-MP479613SWK
CSB-EP479613SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Putative Holliday junction resolvase (yqgF) CSB-YP479614SWK
CSB-EP479614SWK
CSB-BP479614SWK
CSB-MP479614SWK
CSB-EP479614SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona G/U mismatch-specific DNA glycosylase (mug) CSB-YP479615SWK
CSB-EP479615SWK
CSB-BP479615SWK
CSB-MP479615SWK
CSB-EP479615SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0246 protein yaaA (yaaA) CSB-YP479616SWK
CSB-EP479616SWK
CSB-BP479616SWK
CSB-MP479616SWK
CSB-EP479616SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona DNA replication terminus site-binding protein (tus) CSB-YP479617SWK
CSB-EP479617SWK
CSB-BP479617SWK
CSB-MP479617SWK
CSB-EP479617SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Electron transport complex protein RnfE (rnfE), partial CSB-YP479618SWK1
CSB-EP479618SWK1
CSB-BP479618SWK1
CSB-MP479618SWK1
CSB-EP479618SWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona D-tagatose-1,6-bisphosphate aldolase subunit gatZ (gatZ) CSB-YP479619SWK
CSB-EP479619SWK
CSB-BP479619SWK
CSB-MP479619SWK
CSB-EP479619SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 30S ribosomal protein S15 (rpsO) CSB-YP479620SWK
CSB-EP479620SWK
CSB-BP479620SWK
CSB-MP479620SWK
CSB-EP479620SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 50S ribosomal protein L27 (rpmA) CSB-YP479621SWK
CSB-EP479621SWK
CSB-BP479621SWK
CSB-MP479621SWK
CSB-EP479621SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Non-specific ribonucleoside hydrolase rihC (rihC) CSB-YP479622SWK
CSB-EP479622SWK
CSB-BP479622SWK
CSB-MP479622SWK
CSB-EP479622SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Protein fixA (fixA) CSB-YP479623SWK
CSB-EP479623SWK
CSB-BP479623SWK
CSB-MP479623SWK
CSB-EP479623SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona RNA polymerase-associated protein RapA (rapA), partial CSB-YP479624SWK
CSB-EP479624SWK
CSB-BP479624SWK
CSB-MP479624SWK
CSB-EP479624SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Integration host factor subunit alpha (ihfA) CSB-YP479625SWK
CSB-EP479625SWK
CSB-BP479625SWK
CSB-MP479625SWK
CSB-EP479625SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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