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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus UPF0295 protein BCG9842_B4782 (BCG9842_B4782), partial CSB-YP478826BQP1
CSB-EP478826BQP1
CSB-BP478826BQP1
CSB-MP478826BQP1
CSB-EP478826BQP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N utilization substance protein B homolog (nusB) CSB-YP478827BQP
CSB-EP478827BQP
CSB-BP478827BQP
CSB-MP478827BQP
CSB-EP478827BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP478828BQP
CSB-EP478828BQP
CSB-BP478828BQP
CSB-MP478828BQP
CSB-EP478828BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative phosphotransferase BCG9842_B0823 (BCG9842_B0823) CSB-YP478829BQP
CSB-EP478829BQP
CSB-BP478829BQP
CSB-MP478829BQP
CSB-EP478829BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Elongation factor 4 (lepA), partial CSB-YP478830BQP
CSB-EP478830BQP
CSB-BP478830BQP
CSB-MP478830BQP
CSB-EP478830BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP478831BQP
CSB-EP478831BQP
CSB-BP478831BQP
CSB-MP478831BQP
CSB-EP478831BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L19 (rplS) CSB-YP478832BUG
CSB-EP478832BUG
CSB-BP478832BUG
CSB-MP478832BUG
CSB-EP478832BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 10 kDa chaperonin (groS) CSB-YP478833BUG
CSB-EP478833BUG
CSB-BP478833BUG
CSB-MP478833BUG
CSB-EP478833BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Flagellar P-ring protein (flgI) CSB-YP478834BUG
CSB-EP478834BUG
CSB-BP478834BUG
CSB-MP478834BUG
CSB-EP478834BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L25 (rplY) CSB-YP478835BUG
CSB-EP478835BUG
CSB-BP478835BUG
CSB-MP478835BUG
CSB-EP478835BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Ribosome maturation factor RimP (rimP) CSB-YP478836BUG
CSB-EP478836BUG
CSB-BP478836BUG
CSB-MP478836BUG
CSB-EP478836BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Isoleucine--tRNA ligase (ileS), partial CSB-YP478837BUG
CSB-EP478837BUG
CSB-BP478837BUG
CSB-MP478837BUG
CSB-EP478837BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Uridine kinase (udk) CSB-YP478838BUG
CSB-EP478838BUG
CSB-BP478838BUG
CSB-MP478838BUG
CSB-EP478838BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi L-lactate dehydrogenase (ldh) CSB-YP478839BUG
CSB-EP478839BUG
CSB-BP478839BUG
CSB-MP478839BUG
CSB-EP478839BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 30S ribosomal protein S18 (rpsR) CSB-YP478840BUG
CSB-EP478840BUG
CSB-BP478840BUG
CSB-MP478840BUG
CSB-EP478840BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Cytidylate kinase (cmk) CSB-YP478841BUG
CSB-EP478841BUG
CSB-BP478841BUG
CSB-MP478841BUG
CSB-EP478841BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP478842BUG
CSB-EP478842BUG
CSB-BP478842BUG
CSB-MP478842BUG
CSB-EP478842BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 30S ribosomal protein S20 (rpsT) CSB-YP478843BUG
CSB-EP478843BUG
CSB-BP478843BUG
CSB-MP478843BUG
CSB-EP478843BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP478844BUG
CSB-EP478844BUG
CSB-BP478844BUG
CSB-MP478844BUG
CSB-EP478844BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 30S ribosomal protein S9 (rpsI) CSB-YP478845BUG
CSB-EP478845BUG
CSB-BP478845BUG
CSB-MP478845BUG
CSB-EP478845BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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