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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP481860BQO
CSB-EP481860BQO
CSB-BP481860BQO
CSB-MP481860BQO
CSB-EP481860BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative membrane protein insertion efficiency factor (BCB4264_A4918) CSB-YP481861BQO
CSB-EP481861BQO
CSB-BP481861BQO
CSB-MP481861BQO
CSB-EP481861BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) CSB-YP481862BQO
CSB-EP481862BQO
CSB-BP481862BQO
CSB-MP481862BQO
CSB-EP481862BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein tlp (tlp) CSB-YP481863BQO
CSB-EP481863BQO
CSB-BP481863BQO
CSB-MP481863BQO
CSB-EP481863BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP481864BQO
CSB-EP481864BQO
CSB-BP481864BQO
CSB-MP481864BQO
CSB-EP481864BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP481865BQO
CSB-EP481865BQO
CSB-BP481865BQO
CSB-MP481865BQO
CSB-EP481865BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein O (sspO) CSB-YP481866BQO
CSB-EP481866BQO
CSB-BP481866BQO
CSB-MP481866BQO
CSB-EP481866BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holin-like protein CidA (cidA), partial CSB-YP481867BQO1
CSB-EP481867BQO1
CSB-BP481867BQO1
CSB-MP481867BQO1
CSB-EP481867BQO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable endonuclease 4 (nfo) CSB-YP481868BQO
CSB-EP481868BQO
CSB-BP481868BQO
CSB-MP481868BQO
CSB-EP481868BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable rRNA maturation factor (BCB4264_A4420) CSB-YP481869BQO
CSB-EP481869BQO
CSB-BP481869BQO
CSB-MP481869BQO
CSB-EP481869BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chaperone protein DnaJ (dnaJ) CSB-YP481870BQO
CSB-EP481870BQO
CSB-BP481870BQO
CSB-MP481870BQO
CSB-EP481870BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Heat-inducible transcription repressor HrcA (hrcA) CSB-YP481871BQO
CSB-EP481871BQO
CSB-BP481871BQO
CSB-MP481871BQO
CSB-EP481871BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S20 (rpsT) CSB-YP481872BQO
CSB-EP481872BQO
CSB-BP481872BQO
CSB-MP481872BQO
CSB-EP481872BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Potassium-transporting ATPase A chain (kdpA), partial CSB-YP481873BQO
CSB-EP481873BQO
CSB-BP481873BQO
CSB-MP481873BQO
CSB-EP481873BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S15 (rpsO) CSB-YP481874BQO
CSB-EP481874BQO
CSB-BP481874BQO
CSB-MP481874BQO
CSB-EP481874BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP481875BQO
CSB-EP481875BQO
CSB-BP481875BQO
CSB-MP481875BQO
CSB-EP481875BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease HII (rnhB) CSB-YP481876BQO
CSB-EP481876BQO
CSB-BP481876BQO
CSB-MP481876BQO
CSB-EP481876BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative Holliday junction resolvase (BCB4264_A4506) CSB-YP481877BQO
CSB-EP481877BQO
CSB-BP481877BQO
CSB-MP481877BQO
CSB-EP481877BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L27 (rpmA) CSB-YP481878BQO
CSB-EP481878BQO
CSB-BP481878BQO
CSB-MP481878BQO
CSB-EP481878BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamate racemase (murI) CSB-YP481879BQO
CSB-EP481879BQO
CSB-BP481879BQO
CSB-MP481879BQO
CSB-EP481879BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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