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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O45:K1 UPF0434 protein ycaR (ycaR) CSB-YP482661EOK
CSB-EP482661EOK
CSB-BP482661EOK
CSB-MP482661EOK
CSB-EP482661EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Protein CysZ (cysZ), partial CSB-YP482662EOK1
CSB-EP482662EOK1
CSB-BP482662EOK1
CSB-MP482662EOK1
CSB-EP482662EOK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 UPF0370 protein ypfN (ypfN), partial CSB-YP482663EOK
CSB-EP482663EOK
CSB-BP482663EOK
CSB-MP482663EOK
CSB-EP482663EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Histidine--tRNA ligase (hisS) CSB-YP482664EOK
CSB-EP482664EOK
CSB-BP482664EOK
CSB-MP482664EOK
CSB-EP482664EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Peptidase B (pepB) CSB-YP482665EOK
CSB-EP482665EOK
CSB-BP482665EOK
CSB-MP482665EOK
CSB-EP482665EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 L-rhamnose isomerase (rhaA) CSB-YP482666EOK
CSB-EP482666EOK
CSB-BP482666EOK
CSB-MP482666EOK
CSB-EP482666EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 CDP-diacylglycerol pyrophosphatase (cdh), partial CSB-YP482667EOK
CSB-EP482667EOK
CSB-BP482667EOK
CSB-MP482667EOK
CSB-EP482667EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 50S ribosomal protein L31 (rpmE) CSB-YP482668EOK
CSB-EP482668EOK
CSB-BP482668EOK
CSB-MP482668EOK
CSB-EP482668EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Glutamate racemase (murI) CSB-YP482669EOK
CSB-EP482669EOK
CSB-BP482669EOK
CSB-MP482669EOK
CSB-EP482669EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Chaperone modulatory protein CbpM (cbpM) CSB-YP482670EOK
CSB-EP482670EOK
CSB-BP482670EOK
CSB-MP482670EOK
CSB-EP482670EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB (rutB) CSB-YP482671EOK
CSB-EP482671EOK
CSB-BP482671EOK
CSB-MP482671EOK
CSB-EP482671EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Multidrug resistance protein MdtG (mdtG), partial CSB-YP482672EOK
CSB-EP482672EOK
CSB-BP482672EOK
CSB-MP482672EOK
CSB-EP482672EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Iron-binding protein IscA (iscA) CSB-YP482673EOK
CSB-EP482673EOK
CSB-BP482673EOK
CSB-MP482673EOK
CSB-EP482673EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 tRNA (guanine-N (1)-)-methyltransferase CSB-YP482674EOK
CSB-EP482674EOK
CSB-BP482674EOK
CSB-MP482674EOK
CSB-EP482674EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Pantothenate kinase (coaA) CSB-YP482675EOK
CSB-EP482675EOK
CSB-BP482675EOK
CSB-MP482675EOK
CSB-EP482675EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Phosphomethylpyrimidine synthase (thiC) CSB-YP482676EOK
CSB-EP482676EOK
CSB-BP482676EOK
CSB-MP482676EOK
CSB-EP482676EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Isocitrate dehydrogenase kinase/phosphatase (aceK) CSB-YP482677EOK
CSB-EP482677EOK
CSB-BP482677EOK
CSB-MP482677EOK
CSB-EP482677EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 LexA repressor (lexA) CSB-YP482678EOK
CSB-EP482678EOK
CSB-BP482678EOK
CSB-MP482678EOK
CSB-EP482678EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Thiamine kinase (thiK) CSB-YP482679EOK
CSB-EP482679EOK
CSB-BP482679EOK
CSB-MP482679EOK
CSB-EP482679EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Cation/acetate symporter ActP (actP), partial CSB-YP482680EOK
CSB-EP482680EOK
CSB-BP482680EOK
CSB-MP482680EOK
CSB-EP482680EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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