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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus UPF0042 nucleotide-binding protein BCB4264_A5274 (BCB4264_A5274) CSB-YP481880BQO
CSB-EP481880BQO
CSB-BP481880BQO
CSB-MP481880BQO
CSB-EP481880BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphomethylpyrimidine synthase (thiC), partial CSB-YP481881BQO
CSB-EP481881BQO
CSB-BP481881BQO
CSB-MP481881BQO
CSB-EP481881BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus MutS2 protein (mutS2), partial CSB-YP481882BQO
CSB-EP481882BQO
CSB-BP481882BQO
CSB-MP481882BQO
CSB-EP481882BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0173 metal-dependent hydrolase BCB4264_A4722 (BCB4264_A4722) CSB-YP481883BQO
CSB-EP481883BQO
CSB-BP481883BQO
CSB-MP481883BQO
CSB-EP481883BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP481884BQO
CSB-EP481884BQO
CSB-BP481884BQO
CSB-MP481884BQO
CSB-EP481884BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase subunit c (atpE), partial CSB-YP481885BQO1
CSB-EP481885BQO1
CSB-BP481885BQO1
CSB-MP481885BQO1
CSB-EP481885BQO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus HTH-type transcriptional regulator hpr (hpr) CSB-YP481886BQO
CSB-EP481886BQO
CSB-BP481886BQO
CSB-MP481886BQO
CSB-EP481886BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Antiholin-like protein LrgB (lrgB), partial CSB-YP481887BQO1
CSB-EP481887BQO1
CSB-BP481887BQO1
CSB-MP481887BQO1
CSB-EP481887BQO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Nucleoid occlusion protein (noc) CSB-YP481888BQO
CSB-EP481888BQO
CSB-BP481888BQO
CSB-MP481888BQO
CSB-EP481888BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0234 protein BCB4264_A1213 (BCB4264_A1213) CSB-YP481889BQO
CSB-EP481889BQO
CSB-BP481889BQO
CSB-MP481889BQO
CSB-EP481889BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP481890BQO
CSB-EP481890BQO
CSB-BP481890BQO
CSB-MP481890BQO
CSB-EP481890BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Lactate utilization protein C (lutC) CSB-YP481891BQO
CSB-EP481891BQO
CSB-BP481891BQO
CSB-MP481891BQO
CSB-EP481891BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus D-alanine--poly (phosphoribitol) ligase subunit 1, partial CSB-YP481892BQO
CSB-EP481892BQO
CSB-BP481892BQO
CSB-MP481892BQO
CSB-EP481892BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP481893BQO
CSB-EP481893BQO
CSB-BP481893BQO
CSB-MP481893BQO
CSB-EP481893BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP481894BQO
CSB-EP481894BQO
CSB-BP481894BQO
CSB-MP481894BQO
CSB-EP481894BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylglyoxal synthase (mgsA) CSB-YP481895BQO
CSB-EP481895BQO
CSB-BP481895BQO
CSB-MP481895BQO
CSB-EP481895BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cell cycle protein GpsB (gpsB) CSB-YP481896BQO
CSB-EP481896BQO
CSB-BP481896BQO
CSB-MP481896BQO
CSB-EP481896BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA replication and repair protein recF (recF) CSB-YP481897BQO
CSB-EP481897BQO
CSB-BP481897BQO
CSB-MP481897BQO
CSB-EP481897BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Recombination protein RecR (recR) CSB-YP481898BQO
CSB-EP481898BQO
CSB-BP481898BQO
CSB-MP481898BQO
CSB-EP481898BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative septation protein spoVG (spoVG) CSB-YP481899BQO
CSB-EP481899BQO
CSB-BP481899BQO
CSB-MP481899BQO
CSB-EP481899BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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