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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Cyanothece sp. Dihydroorotate dehydrogenase (quinone) CSB-YP482220EPW
CSB-EP482220EPW
CSB-BP482220EPW
CSB-MP482220EPW
CSB-EP482220EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. UPF0367 protein PCC8801_1959 (PCC8801_1959) CSB-YP482221EPW
CSB-EP482221EPW
CSB-BP482221EPW
CSB-MP482221EPW
CSB-EP482221EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Apocytochrome f (petA), partial CSB-YP482222EPW1
CSB-EP482222EPW1
CSB-BP482222EPW1
CSB-MP482222EPW1
CSB-EP482222EPW1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. CinA-like protein (PCC8801_0814) CSB-YP482223EPW
CSB-EP482223EPW
CSB-BP482223EPW
CSB-MP482223EPW
CSB-EP482223EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Probable transcriptional regulatory protein PCC8801_2028 (PCC8801_2028) CSB-YP482224EPW
CSB-EP482224EPW
CSB-BP482224EPW
CSB-MP482224EPW
CSB-EP482224EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L21 (rplU) CSB-YP482225EPW
CSB-EP482225EPW
CSB-BP482225EPW
CSB-MP482225EPW
CSB-EP482225EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Protein RecA (recA) CSB-YP482226EPW
CSB-EP482226EPW
CSB-BP482226EPW
CSB-MP482226EPW
CSB-EP482226EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP482227EPW
CSB-EP482227EPW
CSB-BP482227EPW
CSB-MP482227EPW
CSB-EP482227EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Indole-3-glycerol phosphate synthase (trpC) CSB-YP482228EPW
CSB-EP482228EPW
CSB-BP482228EPW
CSB-MP482228EPW
CSB-EP482228EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. NAD (P)H-quinone oxidoreductase subunit 1, partial CSB-YP482229EPW
CSB-EP482229EPW
CSB-BP482229EPW
CSB-MP482229EPW
CSB-EP482229EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP482230EPW
CSB-EP482230EPW
CSB-BP482230EPW
CSB-MP482230EPW
CSB-EP482230EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP482231EPW1
CSB-EP482231EPW1
CSB-BP482231EPW1
CSB-MP482231EPW1
CSB-EP482231EPW1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP482232EPW
CSB-EP482232EPW
CSB-BP482232EPW
CSB-MP482232EPW
CSB-EP482232EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP482233EPW
CSB-EP482233EPW
CSB-BP482233EPW
CSB-MP482233EPW
CSB-EP482233EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L15 (rplO) CSB-YP482234EPW
CSB-EP482234EPW
CSB-BP482234EPW
CSB-MP482234EPW
CSB-EP482234EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L14 (rplN) CSB-YP482235EPW
CSB-EP482235EPW
CSB-BP482235EPW
CSB-MP482235EPW
CSB-EP482235EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Glycerol kinase (glpK) CSB-YP482236EPW
CSB-EP482236EPW
CSB-BP482236EPW
CSB-MP482236EPW
CSB-EP482236EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. UPF0272 protein PCC8801_2415 (PCC8801_2415) CSB-YP482237EPW
CSB-EP482237EPW
CSB-BP482237EPW
CSB-MP482237EPW
CSB-EP482237EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 30S ribosomal protein S3 (rpsC) CSB-YP482238EPW
CSB-EP482238EPW
CSB-BP482238EPW
CSB-MP482238EPW
CSB-EP482238EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L3 (rplC) CSB-YP482239EPW
CSB-EP482239EPW
CSB-BP482239EPW
CSB-MP482239EPW
CSB-EP482239EPW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過(guò)多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn)，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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