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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia fergusonii Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (arnT), partial CSB-YP482421EOR
CSB-EP482421EOR
CSB-BP482421EOR
CSB-MP482421EOR
CSB-EP482421EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Nucleoside triphosphatase nudI (nudI) CSB-YP482422EOR
CSB-EP482422EOR
CSB-BP482422EOR
CSB-MP482422EOR
CSB-EP482422EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii HMP-PP phosphatase (cof) CSB-YP482423EOR
CSB-EP482423EOR
CSB-BP482423EOR
CSB-MP482423EOR
CSB-EP482423EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP482424EOR
CSB-EP482424EOR
CSB-BP482424EOR
CSB-MP482424EOR
CSB-EP482424EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Shikimate kinase 2 (aroL) CSB-YP482425EOR
CSB-EP482425EOR
CSB-BP482425EOR
CSB-MP482425EOR
CSB-EP482425EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Probable cytosol aminopeptidase (pepA), partial CSB-YP482426EOR
CSB-EP482426EOR
CSB-BP482426EOR
CSB-MP482426EOR
CSB-EP482426EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Uronate isomerase (uxaC) CSB-YP482427EOR
CSB-EP482427EOR
CSB-BP482427EOR
CSB-MP482427EOR
CSB-EP482427EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii UPF0145 protein ybjQ (ybjQ) CSB-YP482428EOR
CSB-EP482428EOR
CSB-BP482428EOR
CSB-MP482428EOR
CSB-EP482428EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Uncharacterized MFS-type transporter ycaD (ycaD), partial CSB-YP482429EOR
CSB-EP482429EOR
CSB-BP482429EOR
CSB-MP482429EOR
CSB-EP482429EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Gamma-glutamyl phosphate reductase (proA) CSB-YP482430EOR
CSB-EP482430EOR
CSB-BP482430EOR
CSB-MP482430EOR
CSB-EP482430EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii RNA pyrophosphohydrolase (rppH) CSB-YP482431EOR
CSB-EP482431EOR
CSB-BP482431EOR
CSB-MP482431EOR
CSB-EP482431EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP482432EOR
CSB-EP482432EOR
CSB-BP482432EOR
CSB-MP482432EOR
CSB-EP482432EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP482433EOR
CSB-EP482433EOR
CSB-BP482433EOR
CSB-MP482433EOR
CSB-EP482433EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Heat shock protein hspQ (hspQ) CSB-YP482434EOR
CSB-EP482434EOR
CSB-BP482434EOR
CSB-MP482434EOR
CSB-EP482434EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii tRNA-modifying protein ygfZ (ygfZ) CSB-YP482435EOR
CSB-EP482435EOR
CSB-BP482435EOR
CSB-MP482435EOR
CSB-EP482435EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Ribose-5-phosphate isomerase A (rpiA) CSB-YP482436EOR
CSB-EP482436EOR
CSB-BP482436EOR
CSB-MP482436EOR
CSB-EP482436EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii tRNA (cmo5U34)-methyltransferase CSB-YP482437EOR
CSB-EP482437EOR
CSB-BP482437EOR
CSB-MP482437EOR
CSB-EP482437EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Protease HtpX (htpX), partial CSB-YP482438EOR1
CSB-EP482438EOR1
CSB-BP482438EOR1
CSB-MP482438EOR1
CSB-EP482438EOR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii S-adenosylmethionine synthase (metK) CSB-YP482439EOR
CSB-EP482439EOR
CSB-BP482439EOR
CSB-MP482439EOR
CSB-EP482439EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii UPF0114 protein YqhA (yqhA), partial CSB-YP482440EOR1
CSB-EP482440EOR1
CSB-BP482440EOR1
CSB-MP482440EOR1
CSB-EP482440EOR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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