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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cyanothece sp. Protein RecA (recA) CSB-YP482281DZZ
CSB-EP482281DZZ
CSB-BP482281DZZ
CSB-MP482281DZZ
CSB-EP482281DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum Biotin synthase (bioB) CSB-YP482282MSY
CSB-EP482282MSY
CSB-BP482282MSY
CSB-MP482282MSY
CSB-EP482282MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum Phosphoglycerate kinase (pgk) CSB-YP482283MSY
CSB-EP482283MSY
CSB-BP482283MSY
CSB-MP482283MSY
CSB-EP482283MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum DNA repair protein recO (recO) CSB-YP482284MSY
CSB-EP482284MSY
CSB-BP482284MSY
CSB-MP482284MSY
CSB-EP482284MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum 5'-nucleotidase surE (surE) CSB-YP482285MSY
CSB-EP482285MSY
CSB-BP482285MSY
CSB-MP482285MSY
CSB-EP482285MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP482286MSY
CSB-EP482286MSY
CSB-BP482286MSY
CSB-MP482286MSY
CSB-EP482286MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP482287MSY
CSB-EP482287MSY
CSB-BP482287MSY
CSB-MP482287MSY
CSB-EP482287MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP482288MSY
CSB-EP482288MSY
CSB-BP482288MSY
CSB-MP482288MSY
CSB-EP482288MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum Protein translocase subunit SecA (secA), partial CSB-YP482289MSY
CSB-EP482289MSY
CSB-BP482289MSY
CSB-MP482289MSY
CSB-EP482289MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP482290MSY
CSB-EP482290MSY
CSB-BP482290MSY
CSB-MP482290MSY
CSB-EP482290MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (bchL) CSB-YP482291MSY
CSB-EP482291MSY
CSB-BP482291MSY
CSB-MP482291MSY
CSB-EP482291MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum S-adenosylmethionine synthase (metK) CSB-YP482292MSY
CSB-EP482292MSY
CSB-BP482292MSY
CSB-MP482292MSY
CSB-EP482292MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum UPF0061 protein Mchl_5348 (Mchl_5348) CSB-YP482293MSY
CSB-EP482293MSY
CSB-BP482293MSY
CSB-MP482293MSY
CSB-EP482293MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum Sec-independent protein translocase protein TatA (tatA) CSB-YP482294MSY
CSB-EP482294MSY
CSB-BP482294MSY
CSB-MP482294MSY
CSB-EP482294MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum FMN-dependent NADH-azoreductase (azoR) CSB-YP482295MSY
CSB-EP482295MSY
CSB-BP482295MSY
CSB-MP482295MSY
CSB-EP482295MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP482296MSY
CSB-EP482296MSY
CSB-BP482296MSY
CSB-MP482296MSY
CSB-EP482296MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum Ribonuclease PH (rph) CSB-YP482297MSY
CSB-EP482297MSY
CSB-BP482297MSY
CSB-MP482297MSY
CSB-EP482297MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum 50S ribosomal protein L16 (rplP) CSB-YP482298MSY
CSB-EP482298MSY
CSB-BP482298MSY
CSB-MP482298MSY
CSB-EP482298MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum 50S ribosomal protein L30 (rpmD) CSB-YP482299MSY
CSB-EP482299MSY
CSB-BP482299MSY
CSB-MP482299MSY
CSB-EP482299MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum Tryptophan synthase beta chain (trpB) CSB-YP482300MSY
CSB-EP482300MSY
CSB-BP482300MSY
CSB-MP482300MSY
CSB-EP482300MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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