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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli HMP-PP phosphatase (cof) CSB-YP482321ENM
CSB-EP482321ENM
CSB-BP482321ENM
CSB-MP482321ENM
CSB-EP482321ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Integration host factor subunit alpha (ihfA) CSB-YP482322ENM
CSB-EP482322ENM
CSB-BP482322ENM
CSB-MP482322ENM
CSB-EP482322ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli NH (3)-dependent NAD (+) synthetase CSB-YP482323ENM
CSB-EP482323ENM
CSB-BP482323ENM
CSB-MP482323ENM
CSB-EP482323ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0756 membrane protein YeaL (yeaL), partial CSB-YP482324ENM1
CSB-EP482324ENM1
CSB-BP482324ENM1
CSB-MP482324ENM1
CSB-EP482324ENM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glyoxylate/hydroxypyruvate reductase B (ghrB) CSB-YP482325ENM
CSB-EP482325ENM
CSB-BP482325ENM
CSB-MP482325ENM
CSB-EP482325ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP482326ENM
CSB-EP482326ENM
CSB-BP482326ENM
CSB-MP482326ENM
CSB-EP482326ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP482327ENM
CSB-EP482327ENM
CSB-BP482327ENM
CSB-MP482327ENM
CSB-EP482327ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Adenine deaminase (ade) CSB-YP482328ENM
CSB-EP482328ENM
CSB-BP482328ENM
CSB-MP482328ENM
CSB-EP482328ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acetyl esterase (aes) CSB-YP482329ENM
CSB-EP482329ENM
CSB-BP482329ENM
CSB-MP482329ENM
CSB-EP482329ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP482330ENM
CSB-EP482330ENM
CSB-BP482330ENM
CSB-MP482330ENM
CSB-EP482330ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Copper homeostasis protein CutC (cutC) CSB-YP482331ENM
CSB-EP482331ENM
CSB-BP482331ENM
CSB-MP482331ENM
CSB-EP482331ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-galactonate dehydratase (dgoD) CSB-YP482332ENM
CSB-EP482332ENM
CSB-BP482332ENM
CSB-MP482332ENM
CSB-EP482332ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional regulator yidZ (yidZ) CSB-YP482333ENM
CSB-EP482333ENM
CSB-BP482333ENM
CSB-MP482333ENM
CSB-EP482333ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Aspartate--ammonia ligase (asnA) CSB-YP482334ENM
CSB-EP482334ENM
CSB-BP482334ENM
CSB-MP482334ENM
CSB-EP482334ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ketol-acid reductoisomerase (ilvC) CSB-YP482335ENM
CSB-EP482335ENM
CSB-BP482335ENM
CSB-MP482335ENM
CSB-EP482335ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable apo-citrate lyase phosphoribosyl-dephospho-CoA transferase (citX) CSB-YP482336ENM
CSB-EP482336ENM
CSB-BP482336ENM
CSB-MP482336ENM
CSB-EP482336ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein dsrB (dsrB) CSB-YP482337ENM
CSB-EP482337ENM
CSB-BP482337ENM
CSB-MP482337ENM
CSB-EP482337ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cobalamin synthase (cobS), partial CSB-YP482338ENM1
CSB-EP482338ENM1
CSB-BP482338ENM1
CSB-MP482338ENM1
CSB-EP482338ENM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP482339ENM
CSB-EP482339ENM
CSB-BP482339ENM
CSB-MP482339ENM
CSB-EP482339ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein FdhE (fdhE) CSB-YP482340ENM
CSB-EP482340ENM
CSB-BP482340ENM
CSB-MP482340ENM
CSB-EP482340ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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