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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli HTH-type transcriptional activator rhaS (rhaS) CSB-YP482341ENM
CSB-EP482341ENM
CSB-BP482341ENM
CSB-MP482341ENM
CSB-EP482341ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Leucine--tRNA ligase (leuS), partial CSB-YP482342ENM
CSB-EP482342ENM
CSB-BP482342ENM
CSB-MP482342ENM
CSB-EP482342ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP482343ENM
CSB-EP482343ENM
CSB-BP482343ENM
CSB-MP482343ENM
CSB-EP482343ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative multidrug resistance protein MdtD (mdtD), partial CSB-YP482344ENM
CSB-EP482344ENM
CSB-BP482344ENM
CSB-MP482344ENM
CSB-EP482344ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA (uracil (54)-C (5))-methyltransferase CSB-YP482345ENM
CSB-EP482345ENM
CSB-BP482345ENM
CSB-MP482345ENM
CSB-EP482345ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Thiazole synthase (thiG) CSB-YP482346ENM
CSB-EP482346ENM
CSB-BP482346ENM
CSB-MP482346ENM
CSB-EP482346ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Potassium-transporting ATPase C chain (kdpC), partial CSB-YP482347ENM1
CSB-EP482347ENM1
CSB-BP482347ENM1
CSB-MP482347ENM1
CSB-EP482347ENM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Quinolinate synthase A (nadA) CSB-YP482348ENM
CSB-EP482348ENM
CSB-BP482348ENM
CSB-MP482348ENM
CSB-EP482348ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Elongation factor P-like protein (yeiP) CSB-YP482349ENM
CSB-EP482349ENM
CSB-BP482349ENM
CSB-MP482349ENM
CSB-EP482349ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable malate:quinone oxidoreductase (mqo) CSB-YP482350ENM
CSB-EP482350ENM
CSB-BP482350ENM
CSB-MP482350ENM
CSB-EP482350ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP482351ENM1
CSB-EP482351ENM1
CSB-BP482351ENM1
CSB-MP482351ENM1
CSB-EP482351ENM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH) CSB-YP482352ENM
CSB-EP482352ENM
CSB-BP482352ENM
CSB-MP482352ENM
CSB-EP482352ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli NADH-quinone oxidoreductase subunit C/D (nuoC) CSB-YP482353ENM
CSB-EP482353ENM
CSB-BP482353ENM
CSB-MP482353ENM
CSB-EP482353ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Maltoporin (lamB) CSB-YP482354ENM
CSB-EP482354ENM
CSB-BP482354ENM
CSB-MP482354ENM
CSB-EP482354ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0115 protein yfcN (yfcN) CSB-YP482355ENM
CSB-EP482355ENM
CSB-BP482355ENM
CSB-MP482355ENM
CSB-EP482355ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Divalent-cation tolerance protein CutA (cutA) CSB-YP482356ENM
CSB-EP482356ENM
CSB-BP482356ENM
CSB-MP482356ENM
CSB-EP482356ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP482357ENM
CSB-EP482357ENM
CSB-BP482357ENM
CSB-MP482357ENM
CSB-EP482357ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable L-ascorbate-6-phosphate lactonase ulaG (ulaG) CSB-YP482358ENM
CSB-EP482358ENM
CSB-BP482358ENM
CSB-MP482358ENM
CSB-EP482358ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli PKHD-type hydroxylase ybiX (ybiX) CSB-YP482359ENM
CSB-EP482359ENM
CSB-BP482359ENM
CSB-MP482359ENM
CSB-EP482359ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA ligase (ligA) CSB-YP482360ENM
CSB-EP482360ENM
CSB-BP482360ENM
CSB-MP482360ENM
CSB-EP482360ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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