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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia fergusonii Citrate lyase acyl carrier protein (citD) CSB-YP482481EOR
CSB-EP482481EOR
CSB-BP482481EOR
CSB-MP482481EOR
CSB-EP482481EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii L-arabinose isomerase (araA) CSB-YP482482EOR
CSB-EP482482EOR
CSB-BP482482EOR
CSB-MP482482EOR
CSB-EP482482EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Protein syd (syd) CSB-YP482483EOR
CSB-EP482483EOR
CSB-BP482483EOR
CSB-MP482483EOR
CSB-EP482483EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Glutamate--cysteine ligase (gshA), partial CSB-YP482484EOR
CSB-EP482484EOR
CSB-BP482484EOR
CSB-MP482484EOR
CSB-EP482484EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Regulatory protein recX (recX) CSB-YP482485EOR
CSB-EP482485EOR
CSB-BP482485EOR
CSB-MP482485EOR
CSB-EP482485EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Ribonuclease HII (rnhB) CSB-YP482486EOR
CSB-EP482486EOR
CSB-BP482486EOR
CSB-MP482486EOR
CSB-EP482486EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii UPF0294 protein yafD (yafD) CSB-YP482487EOR
CSB-EP482487EOR
CSB-BP482487EOR
CSB-MP482487EOR
CSB-EP482487EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii [Protein-PII] uridylyltransferase (glnD), partial CSB-YP482488EOR
CSB-EP482488EOR
CSB-BP482488EOR
CSB-MP482488EOR
CSB-EP482488EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP482489EOR
CSB-EP482489EOR
CSB-BP482489EOR
CSB-MP482489EOR
CSB-EP482489EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP482490EOR
CSB-EP482490EOR
CSB-BP482490EOR
CSB-MP482490EOR
CSB-EP482490EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP482491EOR
CSB-EP482491EOR
CSB-BP482491EOR
CSB-MP482491EOR
CSB-EP482491EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP482492EOR
CSB-EP482492EOR
CSB-BP482492EOR
CSB-MP482492EOR
CSB-EP482492EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Dihydrodipicolinate reductase (dapB) CSB-YP482493EOR
CSB-EP482493EOR
CSB-BP482493EOR
CSB-MP482493EOR
CSB-EP482493EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP482494EOR
CSB-EP482494EOR
CSB-BP482494EOR
CSB-MP482494EOR
CSB-EP482494EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O8 50S ribosomal protein L32 (rpmF) CSB-YP482495EOO
CSB-EP482495EOO
CSB-BP482495EOO
CSB-MP482495EOO
CSB-EP482495EOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O8 High frequency lysogenization protein HflD (hflD) CSB-YP482496EOO
CSB-EP482496EOO
CSB-BP482496EOO
CSB-MP482496EOO
CSB-EP482496EOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O8 D-amino acid dehydrogenase small subunit (dadA) CSB-YP482497EOO
CSB-EP482497EOO
CSB-BP482497EOO
CSB-MP482497EOO
CSB-EP482497EOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O8 Adenylyl-sulfate kinase (cysC) CSB-YP482498EOO
CSB-EP482498EOO
CSB-BP482498EOO
CSB-MP482498EOO
CSB-EP482498EOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O8 Enolase (eno) CSB-YP482499EOO
CSB-EP482499EOO
CSB-BP482499EOO
CSB-MP482499EOO
CSB-EP482499EOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O8 L-fucose isomerase (fucI) CSB-YP482500EOO
CSB-EP482500EOO
CSB-BP482500EOO
CSB-MP482500EOO
CSB-EP482500EOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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