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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus laevis Homeobox protein Hox-C6 (hoxc6) CSB-YP010677XBE
CSB-EP010677XBE
CSB-BP010677XBE
CSB-MP010677XBE
CSB-EP010677XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Translation initiation factor IF-3 (infC) CSB-YP015166GFM
CSB-EP015166GFM
CSB-BP015166GFM
CSB-MP015166GFM
CSB-EP015166GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Thymidine kinase (TK) CSB-BP023577HWY
CSB-MP023577HWY
CSB-EP023577HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Ribonucleoside-diphosphate reductase large subunit (BORF2), partial CSB-YP020518EFA
CSB-EP020518EFA
CSB-BP020518EFA
CSB-MP020518EFA
CSB-EP020518EFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse NADH-ubiquinone oxidoreductase chain 1 (Mtnd1), partial CSB-YP015076MO
CSB-EP015076MO
CSB-BP015076MO
CSB-MP015076MO
CSB-EP015076MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis NADH-ubiquinone oxidoreductase chain 1 (mt-nd1), partial CSB-YP015076XBE
CSB-EP015076XBE
CSB-BP015076XBE
CSB-MP015076XBE
CSB-EP015076XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis NADH-ubiquinone oxidoreductase chain 2 (mt-nd2), partial CSB-YP015077XBE
CSB-EP015077XBE
CSB-BP015077XBE
CSB-MP015077XBE
CSB-EP015077XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster NADH-ubiquinone oxidoreductase chain 2 (mt:ND2), partial CSB-YP015077DLU
CSB-EP015077DLU
CSB-BP015077DLU
CSB-MP015077DLU
CSB-EP015077DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis NADH-ubiquinone oxidoreductase chain 3 (mt-nd3), partial CSB-YP015078XBE1
CSB-EP015078XBE1
CSB-BP015078XBE1
CSB-MP015078XBE1
CSB-EP015078XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079GGZ
CSB-EP015079GGZ
CSB-BP015079GGZ
CSB-MP015079GGZ
CSB-EP015079GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079EXP
CSB-EP015079EXP
CSB-BP015079EXP
CSB-MP015079EXP
CSB-EP015079EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse NADH-ubiquinone oxidoreductase chain 4 (Mtnd4), partial CSB-YP015079MO
CSB-EP015079MO
CSB-BP015079MO
CSB-MP015079MO
CSB-EP015079MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-ubiquinone oxidoreductase chain 4 (ND4), partial CSB-YP015079APK
CSB-EP015079APK
CSB-BP015079APK
CSB-MP015079APK
CSB-EP015079APK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla NADH-ubiquinone oxidoreductase chain 5 (MT-ND5), partial CSB-YP015081GGZ
CSB-EP015081GGZ
CSB-BP015081GGZ
CSB-MP015081GGZ
CSB-EP015081GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis NADH-ubiquinone oxidoreductase chain 5 (mt-nd5), partial CSB-YP015081XBE
CSB-EP015081XBE
CSB-BP015081XBE
CSB-MP015081XBE
CSB-EP015081XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse NADH-ubiquinone oxidoreductase chain 6 (Mtnd6), partial CSB-YP015082MO1
CSB-EP015082MO1
CSB-BP015082MO1
CSB-MP015082MO1
CSB-EP015082MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis ATP synthase protein 8 (mt-atp8), partial CSB-YP015071XBE
CSB-EP015071XBE
CSB-BP015071XBE
CSB-MP015071XBE
CSB-EP015071XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xiphias gladius Superoxide dismutase [Cu-Zn] (sod1) CSB-YP022397XAR
CSB-EP022397XAR
CSB-BP022397XAR
CSB-MP022397XAR
CSB-EP022397XAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Stromelysin-1 (Mmp3) CSB-YP014676RA
CSB-BP014676RA
CSB-MP014676RA
CSB-EP014676RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ig gamma-3 chain C region, partial CSB-YP011158MO1
CSB-EP011158MO1
CSB-BP011158MO1
CSB-MP011158MO1
CSB-EP011158MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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