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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycoplasma genitalium Uncharacterized protein MG144 (MG144), partial CSB-YP343283MLN1
CSB-EP343283MLN1
CSB-BP343283MLN1
CSB-MP343283MLN1
CSB-EP343283MLN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium 50S ribosomal protein L23 (rplW) CSB-YP343284MLN
CSB-EP343284MLN
CSB-BP343284MLN
CSB-MP343284MLN
CSB-EP343284MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium 30S ribosomal protein S17 (rpsQ) CSB-YP343285MLN
CSB-EP343285MLN
CSB-BP343285MLN
CSB-MP343285MLN
CSB-EP343285MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium 50S ribosomal protein L15 (rplO) CSB-YP343286MLN
CSB-EP343286MLN
CSB-BP343286MLN
CSB-MP343286MLN
CSB-EP343286MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium 30S ribosomal protein S11 (rpsK) CSB-YP343287MLN
CSB-EP343287MLN
CSB-BP343287MLN
CSB-MP343287MLN
CSB-EP343287MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Oligoendopeptidase F homolog (pepF), partial CSB-YP343288MLN
CSB-EP343288MLN
CSB-BP343288MLN
CSB-MP343288MLN
CSB-EP343288MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium DNA topoisomerase 4 subunit A (parC), partial CSB-YP343289MLN
CSB-EP343289MLN
CSB-BP343289MLN
CSB-MP343289MLN
CSB-EP343289MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Segregation and condensation protein B (scpB) CSB-YP343290MLN
CSB-EP343290MLN
CSB-BP343290MLN
CSB-MP343290MLN
CSB-EP343290MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP343291MLN
CSB-EP343291MLN
CSB-BP343291MLN
CSB-MP343291MLN
CSB-EP343291MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Protein nrdI (nrdI) CSB-YP343292MLN
CSB-EP343292MLN
CSB-BP343292MLN
CSB-MP343292MLN
CSB-EP343292MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Trigger factor (tig) CSB-YP343293MLN
CSB-EP343293MLN
CSB-BP343293MLN
CSB-MP343293MLN
CSB-EP343293MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Uncharacterized protein MG246 (MG246) CSB-YP343294MLN
CSB-EP343294MLN
CSB-BP343294MLN
CSB-MP343294MLN
CSB-EP343294MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Cysteine--tRNA ligase (cysS) CSB-YP343295MLN
CSB-EP343295MLN
CSB-BP343295MLN
CSB-MP343295MLN
CSB-EP343295MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Dephospho-CoA kinase (coaE) CSB-YP343296MLN
CSB-EP343296MLN
CSB-BP343296MLN
CSB-MP343296MLN
CSB-EP343296MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Proline--tRNA ligase (proS) CSB-YP343297MLN
CSB-EP343297MLN
CSB-BP343297MLN
CSB-MP343297MLN
CSB-EP343297MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Uncharacterized protein MG293 (MG293) CSB-YP343298MLN
CSB-EP343298MLN
CSB-BP343298MLN
CSB-MP343298MLN
CSB-EP343298MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Uncharacterized protein MG316 (MG316), partial CSB-YP343299MLN
CSB-EP343299MLN
CSB-BP343299MLN
CSB-MP343299MLN
CSB-EP343299MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium GTPase Der (der) CSB-YP343300MLN
CSB-EP343300MLN
CSB-BP343300MLN
CSB-MP343300MLN
CSB-EP343300MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Uncharacterized protein MG342 (MG342) CSB-YP343301MLN
CSB-EP343301MLN
CSB-BP343301MLN
CSB-MP343301MLN
CSB-EP343301MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Uncharacterized lipoprotein MG348 (MG348) CSB-YP343302MLN
CSB-EP343302MLN
CSB-BP343302MLN
CSB-MP343302MLN
CSB-EP343302MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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