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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acanthamoeba polyphaga mimivirus Putative HD domain-containing protein L394 (MIMI_L394) CSB-YP719526ADAZ
CSB-EP719526ADAZ
CSB-BP719526ADAZ
CSB-MP719526ADAZ
CSB-EP719526ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R661 (MIMI_R661) CSB-YP719527ADAZ
CSB-EP719527ADAZ
CSB-BP719527ADAZ
CSB-MP719527ADAZ
CSB-EP719527ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L509 (MIMI_L509) CSB-YP719528ADAZ
CSB-EP719528ADAZ
CSB-BP719528ADAZ
CSB-MP719528ADAZ
CSB-EP719528ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R514 (MIMI_R514) CSB-YP719529ADAZ
CSB-EP719529ADAZ
CSB-BP719529ADAZ
CSB-MP719529ADAZ
CSB-EP719529ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative rhomboid protein L523 (MIMI_L523), partial CSB-YP719530ADAZ1
CSB-EP719530ADAZ1
CSB-BP719530ADAZ1
CSB-MP719530ADAZ1
CSB-EP719530ADAZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R530 (MIMI_R530) CSB-YP719531ADAZ
CSB-EP719531ADAZ
CSB-BP719531ADAZ
CSB-MP719531ADAZ
CSB-EP719531ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus HMG box-containing protein R545 (MIMI_R545) CSB-YP719532ADAZ
CSB-EP719532ADAZ
CSB-BP719532ADAZ
CSB-MP719532ADAZ
CSB-EP719532ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L216 (MIMI_L216) CSB-YP719533ADAZ
CSB-EP719533ADAZ
CSB-BP719533ADAZ
CSB-MP719533ADAZ
CSB-EP719533ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative SET domain-containing protein L222 (MIMI_L222) CSB-YP719534ADAZ
CSB-EP719534ADAZ
CSB-BP719534ADAZ
CSB-MP719534ADAZ
CSB-EP719534ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L462 (MIMI_L462) CSB-YP719535ADAZ
CSB-EP719535ADAZ
CSB-BP719535ADAZ
CSB-MP719535ADAZ
CSB-EP719535ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R467 (MIMI_R467) CSB-YP719536ADAZ
CSB-EP719536ADAZ
CSB-BP719536ADAZ
CSB-MP719536ADAZ
CSB-EP719536ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized peptidase C1-like protein L477 (MIMI_L477) CSB-YP719537ADAZ
CSB-EP719537ADAZ
CSB-BP719537ADAZ
CSB-MP719537ADAZ
CSB-EP719537ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R481 (MIMI_R481), partial CSB-YP719538ADAZ
CSB-EP719538ADAZ
CSB-BP719538ADAZ
CSB-MP719538ADAZ
CSB-EP719538ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R819 (MIMI_R819) CSB-YP719539ADAZ
CSB-EP719539ADAZ
CSB-BP719539ADAZ
CSB-MP719539ADAZ
CSB-EP719539ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative truncated GMC-type inactive oxidoreductase R832 (MIMI_R832) CSB-YP719540ADAZ
CSB-EP719540ADAZ
CSB-BP719540ADAZ
CSB-MP719540ADAZ
CSB-EP719540ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Probable lanosterol 14-alpha demethylase (MIMI_L808), partial CSB-YP719541ADAZ
CSB-EP719541ADAZ
CSB-BP719541ADAZ
CSB-MP719541ADAZ
CSB-EP719541ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative BTB/POZ domain-containing protein L834 (MIMI_L834) CSB-YP719542ADAZ
CSB-EP719542ADAZ
CSB-BP719542ADAZ
CSB-MP719542ADAZ
CSB-EP719542ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R406 (MIMI_R406) CSB-YP719543ADAZ
CSB-EP719543ADAZ
CSB-BP719543ADAZ
CSB-MP719543ADAZ
CSB-EP719543ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R413 (MIMI_R413) CSB-YP719544ADAZ
CSB-EP719544ADAZ
CSB-BP719544ADAZ
CSB-MP719544ADAZ
CSB-EP719544ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Nucleoside diphosphate kinase (NDK) CSB-YP719545ADAZ
CSB-EP719545ADAZ
CSB-BP719545ADAZ
CSB-MP719545ADAZ
CSB-EP719545ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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