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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus thuringiensis Elongation factor Ts (tsf) CSB-YP025124BOM
CSB-EP025124BOM
CSB-BP025124BOM
CSB-MP025124BOM
CSB-EP025124BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917BOM
CSB-EP022917BOM
CSB-BP022917BOM
CSB-MP022917BOM
CSB-EP022917BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824BOM
CSB-EP005824BOM
CSB-BP005824BOM
CSB-MP005824BOM
CSB-EP005824BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Lipoyl synthase (lipA) CSB-YP012927BOM
CSB-EP012927BOM
CSB-BP012927BOM
CSB-MP012927BOM
CSB-EP012927BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis ATP synthase subunit alpha (atpA), partial CSB-YP002344BOM
CSB-EP002344BOM
CSB-BP002344BOM
CSB-MP002344BOM
CSB-EP002344BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Porphobilinogen deaminase (hemC) CSB-YP010524CWC
CSB-EP010524CWC
CSB-BP010524CWC
CSB-MP010524CWC
CSB-EP010524CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Thymidylate kinase (tmk) CSB-YP007226CWC
CSB-EP007226CWC
CSB-BP007226CWC
CSB-MP007226CWC
CSB-EP007226CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Serine hydroxymethyltransferase (glyA) CSB-YP021274CWC
CSB-EP021274CWC
CSB-BP021274CWC
CSB-MP021274CWC
CSB-EP021274CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Elongation factor G (fusA), partial CSB-YP009373CWC
CSB-EP009373CWC
CSB-BP009373CWC
CSB-MP009373CWC
CSB-EP009373CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus ATP synthase subunit delta (atpH) CSB-YP002355CWC
CSB-EP002355CWC
CSB-BP002355CWC
CSB-MP002355CWC
CSB-EP002355CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Translation initiation factor IF-3 (infC) CSB-YP015166CWC
CSB-EP015166CWC
CSB-BP015166CWC
CSB-MP015166CWC
CSB-EP015166CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cenarchaeum symbiosum Flap endonuclease 1 (fen) CSB-YP008585DRA
CSB-EP008585DRA
CSB-BP008585DRA
CSB-MP008585DRA
CSB-EP008585DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cenarchaeum symbiosum Elongation factor 2 (fusA), partial CSB-YP007434DRA
CSB-EP007434DRA
CSB-BP007434DRA
CSB-MP007434DRA
CSB-EP007434DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cenarchaeum symbiosum Serine hydroxymethyltransferase (glyA) CSB-YP021274DRA
CSB-EP021274DRA
CSB-BP021274DRA
CSB-MP021274DRA
CSB-EP021274DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine CLK4-associating serine/arginine rich protein (CLASRP), partial CSB-YP021149BO
CSB-EP021149BO
CSB-BP021149BO
CSB-MP021149BO
CSB-EP021149BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis EF-hand calcium-binding domain-containing protein 4A (efcab4a) CSB-YP007443XBF
CSB-EP007443XBF
CSB-BP007443XBF
CSB-MP007443XBF
CSB-EP007443XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Laminin subunit gamma-1 (lamc1), partial CSB-YP012735XBF
CSB-EP012735XBF
CSB-BP012735XBF
CSB-MP012735XBF
CSB-EP012735XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis LYR motif-containing protein 7 (lyrm7) CSB-YP013276XBF
CSB-EP013276XBF
CSB-BP013276XBF
CSB-MP013276XBF
CSB-EP013276XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein FAM160B2 (fam160b2), partial CSB-YP008108DIL
CSB-EP008108DIL
CSB-BP008108DIL
CSB-MP008108DIL
CSB-EP008108DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Sterol regulatory element-binding protein cleavage-activating protein (scap), partial CSB-YP020758XBE
CSB-EP020758XBE
CSB-BP020758XBE
CSB-MP020758XBE
CSB-EP020758XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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