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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Leifsonia xyli subsp. xyli Dephospho-CoA kinase (coaE) CSB-YP724431LAAC
CSB-EP724431LAAC
CSB-BP724431LAAC
CSB-MP724431LAAC
CSB-EP724431LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Methionyl-tRNA formyltransferase (fmt) CSB-YP724432LAAC
CSB-EP724432LAAC
CSB-BP724432LAAC
CSB-MP724432LAAC
CSB-EP724432LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Elongation factor P (efp) CSB-YP724433LAAC
CSB-EP724433LAAC
CSB-BP724433LAAC
CSB-MP724433LAAC
CSB-EP724433LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP724434LAAC
CSB-EP724434LAAC
CSB-BP724434LAAC
CSB-MP724434LAAC
CSB-EP724434LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Probable cytosol aminopeptidase (pepA) CSB-YP724435LAAC
CSB-EP724435LAAC
CSB-BP724435LAAC
CSB-MP724435LAAC
CSB-EP724435LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Ribonuclease 3 (rnc) CSB-YP724436LAAC
CSB-EP724436LAAC
CSB-BP724436LAAC
CSB-MP724436LAAC
CSB-EP724436LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli 30S ribosomal protein S15 (rpsO) CSB-YP724437LAAC
CSB-EP724437LAAC
CSB-BP724437LAAC
CSB-MP724437LAAC
CSB-EP724437LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli 50S ribosomal protein L21 (rplU) CSB-YP724438LAAC
CSB-EP724438LAAC
CSB-BP724438LAAC
CSB-MP724438LAAC
CSB-EP724438LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Ribosome-binding factor A (rbfA) CSB-YP724439LAAC
CSB-EP724439LAAC
CSB-BP724439LAAC
CSB-MP724439LAAC
CSB-EP724439LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Homoserine kinase (thrB) CSB-YP724440LAAC
CSB-EP724440LAAC
CSB-BP724440LAAC
CSB-MP724440LAAC
CSB-EP724440LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Acetylglutamate kinase (argB) CSB-YP724441LAAC
CSB-EP724441LAAC
CSB-BP724441LAAC
CSB-MP724441LAAC
CSB-EP724441LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Protein translocase subunit SecA (secA), partial CSB-YP724442LAAC
CSB-EP724442LAAC
CSB-BP724442LAAC
CSB-MP724442LAAC
CSB-EP724442LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Probable malate:quinone oxidoreductase (mqo) CSB-YP724443LAAC
CSB-EP724443LAAC
CSB-BP724443LAAC
CSB-MP724443LAAC
CSB-EP724443LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Serine--tRNA ligase (serS) CSB-YP724444LAAC
CSB-EP724444LAAC
CSB-BP724444LAAC
CSB-MP724444LAAC
CSB-EP724444LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Glycine--tRNA ligase (glyQS) CSB-YP724445LAAC
CSB-EP724445LAAC
CSB-BP724445LAAC
CSB-MP724445LAAC
CSB-EP724445LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sodium/hydrogen exchanger 4 (SLC9A4), partial CSB-YP724446HU
CSB-EP724446HU
CSB-BP724446HU
CSB-MP724446HU
CSB-EP724446HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila CinA-like protein (DP2957) CSB-YP724447DAAA
CSB-EP724447DAAA
CSB-BP724447DAAA
CSB-MP724447DAAA
CSB-EP724447DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Probable transcriptional regulatory protein DP2908 (DP2908) CSB-YP724448DAAA
CSB-EP724448DAAA
CSB-BP724448DAAA
CSB-MP724448DAAA
CSB-EP724448DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP724449DAAA
CSB-EP724449DAAA
CSB-BP724449DAAA
CSB-MP724449DAAA
CSB-EP724449DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila UPF0102 protein DP2807 (DP2807) CSB-YP724450DAAA
CSB-EP724450DAAA
CSB-BP724450DAAA
CSB-MP724450DAAA
CSB-EP724450DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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