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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Kluyveromyces lactis Serine/threonine-protein kinase SSN3 (SSN3), partial CSB-YP724751KBK
CSB-EP724751KBK
CSB-BP724751KBK
CSB-MP724751KBK
CSB-EP724751KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Protein FYV8 (FYV8), partial CSB-YP724752KBK
CSB-EP724752KBK
CSB-BP724752KBK
CSB-MP724752KBK
CSB-EP724752KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis D-tyrosyl-tRNA (Tyr) deacylase (DTD1) CSB-YP724753KBK
CSB-EP724753KBK
CSB-BP724753KBK
CSB-MP724753KBK
CSB-EP724753KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Altered inheritance of mitochondria protein 41, mitochondrial (AIM41) CSB-YP724754KBK
CSB-EP724754KBK
CSB-BP724754KBK
CSB-MP724754KBK
CSB-EP724754KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Elongation factor G, mitochondrial (MEF1), partial CSB-YP724755KBK
CSB-EP724755KBK
CSB-BP724755KBK
CSB-MP724755KBK
CSB-EP724755KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Monopolar spindle protein 2 (MPS2), partial CSB-YP724756KBK1
CSB-EP724756KBK1
CSB-BP724756KBK1
CSB-MP724756KBK1
CSB-EP724756KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Increased rDNA silencing protein 4 (IRS4), partial CSB-YP724757KBK
CSB-EP724757KBK
CSB-BP724757KBK
CSB-MP724757KBK
CSB-EP724757KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis DNA mismatch repair protein MSH3 (MSH3), partial CSB-YP724758KBK
CSB-EP724758KBK
CSB-BP724758KBK
CSB-MP724758KBK
CSB-EP724758KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Serine/threonine-protein kinase ATG1 (ATG1), partial CSB-YP724759KBK
CSB-EP724759KBK
CSB-BP724759KBK
CSB-MP724759KBK
CSB-EP724759KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Probable nucleolar complex protein 14 (NOP14), partial CSB-YP724760KBK
CSB-EP724760KBK
CSB-BP724760KBK
CSB-MP724760KBK
CSB-EP724760KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Pre-mRNA-splicing factor CWC2 (CWC2) CSB-YP724761KBK
CSB-EP724761KBK
CSB-BP724761KBK
CSB-MP724761KBK
CSB-EP724761KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Eukaryotic translation initiation factor 3 subunit A (TIF32), partial CSB-YP724762KBK
CSB-EP724762KBK
CSB-BP724762KBK
CSB-MP724762KBK
CSB-EP724762KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Putative tyrosine-protein phosphatase OCA1 (OCA1) CSB-YP724763KBK
CSB-EP724763KBK
CSB-BP724763KBK
CSB-MP724763KBK
CSB-EP724763KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Golgi apparatus membrane protein TVP23 (TVP23), partial CSB-YP724764KBK1
CSB-EP724764KBK1
CSB-BP724764KBK1
CSB-MP724764KBK1
CSB-EP724764KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Ribosome-recycling factor, mitochondrial (RRF1), partial CSB-YP724765KBK
CSB-EP724765KBK
CSB-BP724765KBK
CSB-MP724765KBK
CSB-EP724765KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Protoheme IX farnesyltransferase, mitochondrial (COX10), partial CSB-YP724766KBK1
CSB-EP724766KBK1
CSB-BP724766KBK1
CSB-MP724766KBK1
CSB-EP724766KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Respiratory growth induced protein 1 (RGI1) CSB-YP724767KBK
CSB-EP724767KBK
CSB-BP724767KBK
CSB-MP724767KBK
CSB-EP724767KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis pH-response regulator protein palA/RIM20 (RIM20), partial CSB-YP724768KBK
CSB-EP724768KBK
CSB-BP724768KBK
CSB-MP724768KBK
CSB-EP724768KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Pre-mRNA-splicing factor SPP2 (SPP2) CSB-YP724769KBK
CSB-EP724769KBK
CSB-BP724769KBK
CSB-MP724769KBK
CSB-EP724769KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Transcription initiation factor TFIID subunit 4 (TAF4) CSB-YP724770KBK
CSB-EP724770KBK
CSB-BP724770KBK
CSB-MP724770KBK
CSB-EP724770KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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