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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Erwinia carotovora subsp. atroseptica Arginine exporter protein ArgO (argO), partial CSB-YP724811EAAB1
CSB-EP724811EAAB1
CSB-BP724811EAAB1
CSB-MP724811EAAB1
CSB-EP724811EAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Transaldolase 2 (tal2) CSB-YP724812EAAB
CSB-EP724812EAAB
CSB-BP724812EAAB
CSB-MP724812EAAB
CSB-EP724812EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Dihydrodipicolinate reductase (dapB) CSB-YP724813EAAB
CSB-EP724813EAAB
CSB-BP724813EAAB
CSB-MP724813EAAB
CSB-EP724813EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica RNA polymerase-associated protein RapA (rapA), partial CSB-YP724814EAAB
CSB-EP724814EAAB
CSB-BP724814EAAB
CSB-MP724814EAAB
CSB-EP724814EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica 2-isopropylmalate synthase (leuA), partial CSB-YP724815EAAB
CSB-EP724815EAAB
CSB-BP724815EAAB
CSB-MP724815EAAB
CSB-EP724815EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica D-alanine--D-alanine ligase (ddl) CSB-YP724816EAAB
CSB-EP724816EAAB
CSB-BP724816EAAB
CSB-MP724816EAAB
CSB-EP724816EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Lysophospholipid transporter lplT (lplT), partial CSB-YP724817EAAB
CSB-EP724817EAAB
CSB-BP724817EAAB
CSB-MP724817EAAB
CSB-EP724817EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica 50S ribosomal protein L9 (rplI) CSB-YP724818EAAB
CSB-EP724818EAAB
CSB-BP724818EAAB
CSB-MP724818EAAB
CSB-EP724818EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica DNA ligase (ligA), partial CSB-YP724819EAAB
CSB-EP724819EAAB
CSB-BP724819EAAB
CSB-MP724819EAAB
CSB-EP724819EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP724820EAAB
CSB-EP724820EAAB
CSB-BP724820EAAB
CSB-MP724820EAAB
CSB-EP724820EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Enolase-phosphatase E1 (mtnC) CSB-YP724821EAAB
CSB-EP724821EAAB
CSB-BP724821EAAB
CSB-MP724821EAAB
CSB-EP724821EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Glutamate 5-kinase (proB) CSB-YP724822EAAB
CSB-EP724822EAAB
CSB-BP724822EAAB
CSB-MP724822EAAB
CSB-EP724822EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica 30S ribosomal protein S16 (rpsP) CSB-YP724823EAAB
CSB-EP724823EAAB
CSB-BP724823EAAB
CSB-MP724823EAAB
CSB-EP724823EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica S-adenosylmethionine decarboxylase proenzyme (speD) CSB-YP724824EAAB
CSB-EP724824EAAB
CSB-BP724824EAAB
CSB-MP724824EAAB
CSB-EP724824EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP724825EAAB
CSB-EP724825EAAB
CSB-BP724825EAAB
CSB-MP724825EAAB
CSB-EP724825EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Ribonuclease 3 (rnc) CSB-YP724826EAAB
CSB-EP724826EAAB
CSB-BP724826EAAB
CSB-MP724826EAAB
CSB-EP724826EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica HTH-type transcriptional regulator iscR (iscR) CSB-YP724827EAAB
CSB-EP724827EAAB
CSB-BP724827EAAB
CSB-MP724827EAAB
CSB-EP724827EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Cytoskeleton protein rodZ (rodZ), partial CSB-YP724828EAAB
CSB-EP724828EAAB
CSB-BP724828EAAB
CSB-MP724828EAAB
CSB-EP724828EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Probable lipid kinase YegS-like (ECA3194) CSB-YP724829EAAB
CSB-EP724829EAAB
CSB-BP724829EAAB
CSB-MP724829EAAB
CSB-EP724829EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Cysteine--tRNA ligase (cysS) CSB-YP724830EAAB
CSB-EP724830EAAB
CSB-BP724830EAAB
CSB-MP724830EAAB
CSB-EP724830EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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