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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Haloarcula marismortui Alanine--tRNA ligase (alaS), partial CSB-YP726071HTJ
CSB-EP726071HTJ
CSB-BP726071HTJ
CSB-MP726071HTJ
CSB-EP726071HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Probable GTP-binding protein EngB (engB) CSB-YP726072HTJ
CSB-EP726072HTJ
CSB-BP726072HTJ
CSB-MP726072HTJ
CSB-EP726072HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui UPF0278 protein rrnAC2265 (rrnAC2265) CSB-YP726073HTJ
CSB-EP726073HTJ
CSB-BP726073HTJ
CSB-MP726073HTJ
CSB-EP726073HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Preprotein translocase subunit SecG (secG), partial CSB-YP726074HTJ
CSB-EP726074HTJ
CSB-BP726074HTJ
CSB-MP726074HTJ
CSB-EP726074HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Methionine--tRNA ligase (metG), partial CSB-YP726075HTJ
CSB-EP726075HTJ
CSB-BP726075HTJ
CSB-MP726075HTJ
CSB-EP726075HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Probable tRNA pseudouridine synthase D (truD) CSB-YP726076HTJ
CSB-EP726076HTJ
CSB-BP726076HTJ
CSB-MP726076HTJ
CSB-EP726076HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Putative pterin-4-alpha-carbinolamine dehydratase (rrnAC1707) CSB-YP726077HTJ
CSB-EP726077HTJ
CSB-BP726077HTJ
CSB-MP726077HTJ
CSB-EP726077HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Cell division control protein 6 homolog 3 (cdc6-3) CSB-YP726078HTJ
CSB-EP726078HTJ
CSB-BP726078HTJ
CSB-MP726078HTJ
CSB-EP726078HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui 30S ribosomal protein S3Ae (rps3Ae) CSB-YP726079HTJ
CSB-EP726079HTJ
CSB-BP726079HTJ
CSB-MP726079HTJ
CSB-EP726079HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Cysteine--tRNA ligase (cysS) CSB-YP726080HTJ
CSB-EP726080HTJ
CSB-BP726080HTJ
CSB-MP726080HTJ
CSB-EP726080HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Aspartate carbamoyltransferase (pyrB) CSB-YP726081HTJ
CSB-EP726081HTJ
CSB-BP726081HTJ
CSB-MP726081HTJ
CSB-EP726081HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Acylphosphatase (acyP) CSB-YP726082HTJ
CSB-EP726082HTJ
CSB-BP726082HTJ
CSB-MP726082HTJ
CSB-EP726082HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Serine hydroxymethyltransferase (glyA) CSB-YP726083HTJ
CSB-EP726083HTJ
CSB-BP726083HTJ
CSB-MP726083HTJ
CSB-EP726083HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Protein pelota homolog (pelA) CSB-YP726084HTJ
CSB-EP726084HTJ
CSB-BP726084HTJ
CSB-MP726084HTJ
CSB-EP726084HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui tRNA (cytidine (56)-2'-O)-methyltransferase (rrnAC0857) CSB-YP726085HTJ
CSB-EP726085HTJ
CSB-BP726085HTJ
CSB-MP726085HTJ
CSB-EP726085HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Anthranilate synthase component 1 1 (trpE1) CSB-YP726086HTJ
CSB-EP726086HTJ
CSB-BP726086HTJ
CSB-MP726086HTJ
CSB-EP726086HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Tyrosine--tRNA ligase (tyrS) CSB-YP726087HTJ
CSB-EP726087HTJ
CSB-BP726087HTJ
CSB-MP726087HTJ
CSB-EP726087HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP726088HTJ
CSB-EP726088HTJ
CSB-BP726088HTJ
CSB-MP726088HTJ
CSB-EP726088HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui UPF0212 protein rrnAC0441 (rrnAC0441) CSB-YP726089HTJ
CSB-EP726089HTJ
CSB-BP726089HTJ
CSB-MP726089HTJ
CSB-EP726089HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui UPF0173 metal-dependent hydrolase rrnAC0300 (rrnAC0300) CSB-YP726090HTJ
CSB-EP726090HTJ
CSB-BP726090HTJ
CSB-MP726090HTJ
CSB-EP726090HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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