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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pongo abelii Leucine-rich repeat protein SHOC-2 (SHOC2), partial CSB-YP725374PYX
CSB-EP725374PYX
CSB-BP725374PYX
CSB-MP725374PYX
CSB-EP725374PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii RNA polymerase II-associated factor 1 homolog (PAF1), partial CSB-YP725375PYX
CSB-EP725375PYX
CSB-BP725375PYX
CSB-MP725375PYX
CSB-EP725375PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Fibroblast growth factor 7 (FGF7) CSB-YP725376PYX
CSB-EP725376PYX
CSB-BP725376PYX
CSB-MP725376PYX
CSB-EP725376PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii 60S ribosomal protein L36 (RPL36) CSB-YP725377PYX
CSB-EP725377PYX
CSB-BP725377PYX
CSB-MP725377PYX
CSB-EP725377PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Prohibitin-2 (PHB2) CSB-YP725378PYX
CSB-EP725378PYX
CSB-BP725378PYX
CSB-MP725378PYX
CSB-EP725378PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii 39S ribosomal protein L53, mitochondrial (MRPL53), partial CSB-YP725379PYX
CSB-EP725379PYX
CSB-BP725379PYX
CSB-MP725379PYX
CSB-EP725379PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Mitochondrial tRNA-specific 2-thiouridylase 1 (TRMU) CSB-YP725380PYX
CSB-EP725380PYX
CSB-BP725380PYX
CSB-MP725380PYX
CSB-EP725380PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Netrin-4 (NTN4), partial CSB-YP725381PYX
CSB-EP725381PYX
CSB-BP725381PYX
CSB-MP725381PYX
CSB-EP725381PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Myosin regulatory light polypeptide 9 (MYL9) CSB-YP725382PYX
CSB-EP725382PYX
CSB-BP725382PYX
CSB-MP725382PYX
CSB-EP725382PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii RNA-binding motif, single-stranded-interacting protein 3 (RBMS3) CSB-YP725383PYX
CSB-EP725383PYX
CSB-BP725383PYX
CSB-MP725383PYX
CSB-EP725383PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii 60S ribosomal protein L37a (RPL37A) CSB-YP725384PYX
CSB-EP725384PYX
CSB-BP725384PYX
CSB-MP725384PYX
CSB-EP725384PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Uncharacterized protein C19orf21 homolog, partial CSB-YP725385PYX
CSB-EP725385PYX
CSB-BP725385PYX
CSB-MP725385PYX
CSB-EP725385PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Splicing factor 3B subunit 3 (SF3B3), partial CSB-YP725386PYX
CSB-EP725386PYX
CSB-BP725386PYX
CSB-MP725386PYX
CSB-EP725386PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii H (+)/Cl (-) exchange transporter 5 (CLCN5), partial CSB-YP725387PYX
CSB-EP725387PYX
CSB-BP725387PYX
CSB-MP725387PYX
CSB-EP725387PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Fibronectin type-III domain-containing protein 3A (FNDC3A), partial CSB-YP725388PYX
CSB-EP725388PYX
CSB-BP725388PYX
CSB-MP725388PYX
CSB-EP725388PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Macrophage-expressed gene 1 protein (MPEG1), partial CSB-YP725389PYX
CSB-EP725389PYX
CSB-BP725389PYX
CSB-MP725389PYX
CSB-EP725389PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Ubiquitin carboxyl-terminal hydrolase 46 (USP46) CSB-YP725390PYX
CSB-EP725390PYX
CSB-BP725390PYX
CSB-MP725390PYX
CSB-EP725390PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Transmembrane protein 234 (TMEM234), partial CSB-YP725391PYX1
CSB-EP725391PYX1
CSB-BP725391PYX1
CSB-MP725391PYX1
CSB-EP725391PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii 60S ribosomal protein L31 (RPL31) CSB-YP725392PYX
CSB-EP725392PYX
CSB-BP725392PYX
CSB-MP725392PYX
CSB-EP725392PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii UPF0538 protein C2orf76 homolog CSB-YP725393PYX
CSB-EP725393PYX
CSB-BP725393PYX
CSB-MP725393PYX
CSB-EP725393PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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