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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant UDP-glucose 6-dehydrogenase (hasB) CSB-YP726291SMZ
CSB-EP726291SMZ
CSB-BP726291SMZ
CSB-MP726291SMZ
CSB-EP726291SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP726292SMZ
CSB-EP726292SMZ
CSB-BP726292SMZ
CSB-MP726292SMZ
CSB-EP726292SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S4 (rpsD) CSB-YP726293SMZ
CSB-EP726293SMZ
CSB-BP726293SMZ
CSB-MP726293SMZ
CSB-EP726293SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP726294SMZ
CSB-EP726294SMZ
CSB-BP726294SMZ
CSB-MP726294SMZ
CSB-EP726294SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0473 protein M6_Spy1794 (M6_Spy1794) CSB-YP726295SMZ
CSB-EP726295SMZ
CSB-BP726295SMZ
CSB-MP726295SMZ
CSB-EP726295SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Formate--tetrahydrofolate ligase 2 (fhs2), partial CSB-YP726296SMZ
CSB-EP726296SMZ
CSB-BP726296SMZ
CSB-MP726296SMZ
CSB-EP726296SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable dipeptidase B (pepDB) CSB-YP726297SMZ
CSB-EP726297SMZ
CSB-BP726297SMZ
CSB-MP726297SMZ
CSB-EP726297SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant C5a peptidase (scpA), partial CSB-YP726298SMZ
CSB-EP726298SMZ
CSB-BP726298SMZ
CSB-MP726298SMZ
CSB-EP726298SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proline--tRNA ligase (proS), partial CSB-YP726299SMZ
CSB-EP726299SMZ
CSB-BP726299SMZ
CSB-MP726299SMZ
CSB-EP726299SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cysteine--tRNA ligase (cysS) CSB-YP726300SMZ
CSB-EP726300SMZ
CSB-BP726300SMZ
CSB-MP726300SMZ
CSB-EP726300SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tagatose 1,6-diphosphate aldolase 2 (lacD2) CSB-YP726301SMZ
CSB-EP726301SMZ
CSB-BP726301SMZ
CSB-MP726301SMZ
CSB-EP726301SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0356 protein M6_Spy1605 (M6_Spy1605) CSB-YP726302SMZ
CSB-EP726302SMZ
CSB-BP726302SMZ
CSB-MP726302SMZ
CSB-EP726302SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S18 (rpsR) CSB-YP726303SMZ
CSB-EP726303SMZ
CSB-BP726303SMZ
CSB-MP726303SMZ
CSB-EP726303SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor P (efp) CSB-YP726304SMZ
CSB-EP726304SMZ
CSB-BP726304SMZ
CSB-MP726304SMZ
CSB-EP726304SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP726305SMZ
CSB-EP726305SMZ
CSB-BP726305SMZ
CSB-MP726305SMZ
CSB-EP726305SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP726306SMZ
CSB-EP726306SMZ
CSB-BP726306SMZ
CSB-MP726306SMZ
CSB-EP726306SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Galactose-6-phosphate isomerase subunit lacB 1 (lacB1) CSB-YP726307SMZ
CSB-EP726307SMZ
CSB-BP726307SMZ
CSB-MP726307SMZ
CSB-EP726307SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Spy1430 (M6_Spy1430) CSB-YP726308SMZ
CSB-EP726308SMZ
CSB-BP726308SMZ
CSB-MP726308SMZ
CSB-EP726308SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0398 protein M6_Spy1399 (M6_Spy1399) CSB-YP726309SMZ
CSB-EP726309SMZ
CSB-BP726309SMZ
CSB-MP726309SMZ
CSB-EP726309SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cysteine synthase (cysK) CSB-YP726310SMZ
CSB-EP726310SMZ
CSB-BP726310SMZ
CSB-MP726310SMZ
CSB-EP726310SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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