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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acanthamoeba polyphaga mimivirus Putative serine/threonine-protein kinase L232 (MIMI_L232), partial CSB-YP726011ADAZ
CSB-EP726011ADAZ
CSB-BP726011ADAZ
CSB-MP726011ADAZ
CSB-EP726011ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L461 (MIMI_L461) CSB-YP726012ADAZ
CSB-EP726012ADAZ
CSB-BP726012ADAZ
CSB-MP726012ADAZ
CSB-EP726012ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R468 (MIMI_R468), partial CSB-YP726013ADAZ1
CSB-EP726013ADAZ1
CSB-BP726013ADAZ1
CSB-MP726013ADAZ1
CSB-EP726013ADAZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative AAA family ATPase R476 (MIMI_R476), partial CSB-YP726014ADAZ
CSB-EP726014ADAZ
CSB-BP726014ADAZ
CSB-MP726014ADAZ
CSB-EP726014ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R474 (MIMI_R474), partial CSB-YP726015ADAZ1
CSB-EP726015ADAZ1
CSB-BP726015ADAZ1
CSB-MP726015ADAZ1
CSB-EP726015ADAZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus DNA topoisomerase 2 (TOP2), partial CSB-YP726016ADAZ
CSB-EP726016ADAZ
CSB-BP726016ADAZ
CSB-MP726016ADAZ
CSB-EP726016ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R489 (MIMI_R489) CSB-YP726017ADAZ
CSB-EP726017ADAZ
CSB-BP726017ADAZ
CSB-MP726017ADAZ
CSB-EP726017ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Eukaryotic translation initiation factor 4E homolog (MIMI_L496) CSB-YP726018ADAZ
CSB-EP726018ADAZ
CSB-BP726018ADAZ
CSB-MP726018ADAZ
CSB-EP726018ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R816 (MIMI_R816) CSB-YP726019ADAZ
CSB-EP726019ADAZ
CSB-BP726019ADAZ
CSB-MP726019ADAZ
CSB-EP726019ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L823 (MIMI_L823) CSB-YP726020ADAZ
CSB-EP726020ADAZ
CSB-BP726020ADAZ
CSB-MP726020ADAZ
CSB-EP726020ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative truncated GMC-type inactive oxidoreductase R833 (MIMI_R833) CSB-YP726021ADAZ
CSB-EP726021ADAZ
CSB-BP726021ADAZ
CSB-MP726021ADAZ
CSB-EP726021ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R839 (MIMI_R839) CSB-YP726022ADAZ
CSB-EP726022ADAZ
CSB-BP726022ADAZ
CSB-MP726022ADAZ
CSB-EP726022ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L862 (MIMI_L862) CSB-YP726023ADAZ
CSB-EP726023ADAZ
CSB-BP726023ADAZ
CSB-MP726023ADAZ
CSB-EP726023ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein L399 (MIMI_L399) CSB-YP726024ADAZ
CSB-EP726024ADAZ
CSB-BP726024ADAZ
CSB-MP726024ADAZ
CSB-EP726024ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative FNIP repeat-containing protein L415 (MIMI_L415) CSB-YP726025ADAZ
CSB-EP726025ADAZ
CSB-BP726025ADAZ
CSB-MP726025ADAZ
CSB-EP726025ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R424 (MIMI_R424) CSB-YP726026ADAZ
CSB-EP726026ADAZ
CSB-BP726026ADAZ
CSB-MP726026ADAZ
CSB-EP726026ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Robin (MIMI_R420) CSB-YP726027ADAZ
CSB-EP726027ADAZ
CSB-BP726027ADAZ
CSB-MP726027ADAZ
CSB-EP726027ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R428 (MIMI_R428) CSB-YP726028ADAZ
CSB-EP726028ADAZ
CSB-BP726028ADAZ
CSB-MP726028ADAZ
CSB-EP726028ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Thioredoxin domain-containing protein R443 (MIMI_R443), partial CSB-YP726029ADAZ
CSB-EP726029ADAZ
CSB-BP726029ADAZ
CSB-MP726029ADAZ
CSB-EP726029ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Uncharacterized protein R455 (MIMI_R455), partial CSB-YP726030ADAZ1
CSB-EP726030ADAZ1
CSB-BP726030ADAZ1
CSB-MP726030ADAZ1
CSB-EP726030ADAZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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