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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mannheimia succiniciproducens UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP727542MAAB
CSB-EP727542MAAB
CSB-BP727542MAAB
CSB-MP727542MAAB
CSB-EP727542MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens D-alanine--D-alanine ligase (ddl) CSB-YP727543MAAB
CSB-EP727543MAAB
CSB-BP727543MAAB
CSB-MP727543MAAB
CSB-EP727543MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Methionine--tRNA ligase (metG), partial CSB-YP727544MAAB
CSB-EP727544MAAB
CSB-BP727544MAAB
CSB-MP727544MAAB
CSB-EP727544MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Deoxycytidine triphosphate deaminase (dcd) CSB-YP727545MAAB
CSB-EP727545MAAB
CSB-BP727545MAAB
CSB-MP727545MAAB
CSB-EP727545MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP727546MAAB1
CSB-EP727546MAAB1
CSB-BP727546MAAB1
CSB-MP727546MAAB1
CSB-EP727546MAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens GTP cyclohydrolase-2 (ribA) CSB-YP727547MAAB
CSB-EP727547MAAB
CSB-BP727547MAAB
CSB-MP727547MAAB
CSB-EP727547MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Peptide chain release factor 3 (prfC), partial CSB-YP727548MAAB
CSB-EP727548MAAB
CSB-BP727548MAAB
CSB-MP727548MAAB
CSB-EP727548MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens tRNA pseudouridine synthase B (truB) CSB-YP727549MAAB
CSB-EP727549MAAB
CSB-BP727549MAAB
CSB-MP727549MAAB
CSB-EP727549MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP727550MAAB
CSB-EP727550MAAB
CSB-BP727550MAAB
CSB-MP727550MAAB
CSB-EP727550MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens UPF0299 membrane protein MS1271 (MS1271), partial CSB-YP727551MAAB1
CSB-EP727551MAAB1
CSB-BP727551MAAB1
CSB-MP727551MAAB1
CSB-EP727551MAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ), partial CSB-YP727552MAAB
CSB-EP727552MAAB
CSB-BP727552MAAB
CSB-MP727552MAAB
CSB-EP727552MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Glucose-6-phosphate isomerase (pgi), partial CSB-YP727553MAAB
CSB-EP727553MAAB
CSB-BP727553MAAB
CSB-MP727553MAAB
CSB-EP727553MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Macrolide export ATP-binding/permease protein MacB (macB), partial CSB-YP727554MAAB1
CSB-EP727554MAAB1
CSB-BP727554MAAB1
CSB-MP727554MAAB1
CSB-EP727554MAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Glycogen synthase (glgA) CSB-YP727555MAAB
CSB-EP727555MAAB
CSB-BP727555MAAB
CSB-MP727555MAAB
CSB-EP727555MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Chromosome partition protein MukB (mukB), partial CSB-YP727556MAAB
CSB-EP727556MAAB
CSB-BP727556MAAB
CSB-MP727556MAAB
CSB-EP727556MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP727557MAAB
CSB-EP727557MAAB
CSB-BP727557MAAB
CSB-MP727557MAAB
CSB-EP727557MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Asparagine--tRNA ligase (asnS) CSB-YP727558MAAB
CSB-EP727558MAAB
CSB-BP727558MAAB
CSB-MP727558MAAB
CSB-EP727558MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Chaperone protein DnaK (dnaK), partial CSB-YP727559MAAB
CSB-EP727559MAAB
CSB-BP727559MAAB
CSB-MP727559MAAB
CSB-EP727559MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP727560MAAB
CSB-EP727560MAAB
CSB-BP727560MAAB
CSB-MP727560MAAB
CSB-EP727560MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia succiniciproducens Amino-acid acetyltransferase (argA) CSB-YP727561MAAB
CSB-EP727561MAAB
CSB-BP727561MAAB
CSB-MP727561MAAB
CSB-EP727561MAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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